Evaluation of CD49f As a Novel Surface Marker to Identify Functional Adipose-derived Mesenchymal Stem Cell Subset

Overview

Journal Cell Prolif

Date 2021 Mar 11

PMID 33704842

Citations 10

Authors

Kangkang Zha

Xu Li

Guangzhao Tian

Zhen Yang

Zhiqiang Sun

Yu Yang

Fu Wei

Bo Huang

Shuangpeng Jiang

Hao Li

Xiang Sui

Shuyun Liu

Quanyi Guo

Affiliations

Soon will be listed here.

Abstract

Objectives: CD49f is expressed on a variety of stem cells and has certain effects on their cytological functions, such as proliferation and differentiation potential. However, whether CD49f is expressed on the surface of adipose tissue-derived mesenchymal stem cells (ADSCs) and its effect on ADSCs has not been clarified.

Materials And Methods: The effects of in vitro culture passage and inflammatory factor treatment on CD49f expression and the adhesion ability of ADSCs from mice and rats were investigated. CD49f cells were selected from rat ADSCs (rADSCs) by magnetic-activated cell sorting (MACS), and the cellular functions of CD49f ADSCs and unsorted ADSCs, including their clonogenic, proliferation, adipogenic and osteogenic differentiation, migration and anti-apoptotic capacities, were compared.

Results: CD49f expression and the adhesion ability of ADSCs decreased with increasing in vitro culture passage number. TNF-α and IFN-γ treatment decreased CD49f expression but increased the adhesion ability of ADSCs. After CD49f was blocked with an anti-CD49f antibody, the adhesion ability of ADSCs was decreased. No significant difference in clonogenic activity was observed between unsorted ADSCs and CD49f ADSCs. CD49f ADSCs had greater proliferation, adipogenic and osteogenic differentiation, migration and anti-apoptotic capacities than unsorted ADSCs.

Conclusion: In the current study, the expression of CD49f on ADSCs was identified for the first time. The expression of CD49f on ADSCs was influenced by in vitro culture passage number and inflammatory factor treatment. Compared with unsorted ADSCs, CD49f ADSCs exhibited superior cellular functions, thus may have great application value in mesenchymal stem cell (MSC)-based therapies.

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References

Li Z, Zhang C, Weiner L, Zhang Y, Zhong J . Molecular characterization of heterogeneous mesenchymal stem cells with single-cell transcriptomes. Biotechnol Adv. 2012; 31(2):312-7. PMC: 3767306. DOI: 10.1016/j.biotechadv.2012.12.003. View

Yang Z, Dong P, Fu X, Li Q, Ma S, Wu D . CD49f Acts as an Inflammation Sensor to Regulate Differentiation, Adhesion, and Migration of Human Mesenchymal Stem Cells. Stem Cells. 2015; 33(9):2798-810. DOI: 10.1002/stem.2063. View

Fu Q, Tang N, Zhang Q, Liu Y, Peng J, Fang N . Preclinical Study of Cell Therapy for Osteonecrosis of the Femoral Head with Allogenic Peripheral Blood-Derived Mesenchymal Stem Cells. Yonsei Med J. 2016; 57(4):1006-15. PMC: 4951443. DOI: 10.3349/ymj.2016.57.4.1006. View

Wofford A, Bow A, Newby S, Brooks S, Rodriguez R, Masi T . Human Fat-Derived Mesenchymal Stem Cells Xenogenically Implanted in a Rat Model Show Enhanced New Bone Formation in Maxillary Alveolar Tooth Defects. Stem Cells Int. 2020; 2020:8142938. PMC: 7201503. DOI: 10.1155/2020/8142938. View

Podesta M, Remuzzi G, Casiraghi F . Mesenchymal Stromal Cells for Transplant Tolerance. Front Immunol. 2019; 10:1287. PMC: 6559333. DOI: 10.3389/fimmu.2019.01287. View

Yang Z, Ma S, Cao R, Liu L, Cao C, Shen Z . CD49f Defines a Distinct Skin Mesenchymal Stem Cell Population Capable of Hair Follicle Epithelial Cell Maintenance. J Invest Dermatol. 2019; 140(3):544-555.e9. DOI: 10.1016/j.jid.2019.08.442. View

Lu Z, Chen W, Li Y, Li L, Zhang H, Pang Y . TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways. Mol Med Rep. 2016; 14(1):643-8. PMC: 4918617. DOI: 10.3892/mmr.2016.5314. View

Shi L, Fu S, Fahim S, Pan S, Lina H, Mu X . TNF-alpha stimulation increases dental pulp stem cell migration in vitro through integrin alpha-6 subunit upregulation. Arch Oral Biol. 2017; 75:48-54. DOI: 10.1016/j.archoralbio.2016.12.005. View

Cil N, Yaka M, Unal M, Dodurga Y, Tan S, Secme M . Adipose derived mesenchymal stem cell treatment in experimental asherman syndrome induced rats. Mol Biol Rep. 2020; 47(6):4541-4552. DOI: 10.1007/s11033-020-05505-4. View

10.

Zhong Z, Chen A, Fa Z, Ding Z, Xiao L, Wu G . Bone marrow mesenchymal stem cells upregulate PI3K/AKT pathway and down-regulate NF-κB pathway by secreting glial cell-derived neurotrophic factors to regulate microglial polarization and alleviate deafferentation pain in rats. Neurobiol Dis. 2020; 143:104945. DOI: 10.1016/j.nbd.2020.104945. View

11.

Ren G, Zhao X, Zhang L, Zhang J, LHuillier A, Ling W . Inflammatory cytokine-induced intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in mesenchymal stem cells are critical for immunosuppression. J Immunol. 2010; 184(5):2321-8. PMC: 2881946. DOI: 10.4049/jimmunol.0902023. View

12.

Przekora A, Juszkiewicz L . The Effect of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells' Therapy in the Treatment of Chronic Posttraumatic Spinal Cord Injury in a Domestic Ferret Patient. Cell Transplant. 2020; 29:963689720928982. PMC: 7563821. DOI: 10.1177/0963689720928982. View

13.

Notta F, Doulatov S, Laurenti E, Poeppl A, Jurisica I, Dick J . Isolation of single human hematopoietic stem cells capable of long-term multilineage engraftment. Science. 2011; 333(6039):218-21. DOI: 10.1126/science.1201219. View

14.

Phinney D . Functional heterogeneity of mesenchymal stem cells: implications for cell therapy. J Cell Biochem. 2012; 113(9):2806-12. DOI: 10.1002/jcb.24166. View

15.

Li X, Guo W, Zha K, Jing X, Wang M, Zhang Y . Enrichment of CD146 Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration. Theranostics. 2019; 9(17):5105-5121. PMC: 6691381. DOI: 10.7150/thno.33904. View

16.

Hu C, Li L . Preconditioning influences mesenchymal stem cell properties in vitro and in vivo. J Cell Mol Med. 2018; 22(3):1428-1442. PMC: 5824372. DOI: 10.1111/jcmm.13492. View

17.

Zhang X, Wang S, Ding X, Guo J, Tian Z . Potential methods for improving the efficacy of mesenchymal stem cells in the treatment of inflammatory bowel diseases. Scand J Immunol. 2020; 92(3):e12897. DOI: 10.1111/sji.12897. View

18.

Wu H, Li J, Xie B, Tian H, Fang S, Jiang S . Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular Restoration. Stem Cells Dev. 2018; 27(9):612-623. DOI: 10.1089/scd.2017.0271. View

19.

Costa L, Eiro N, Fraile M, Gonzalez L, Saa J, Garcia-Portabella P . Functional heterogeneity of mesenchymal stem cells from natural niches to culture conditions: implications for further clinical uses. Cell Mol Life Sci. 2020; 78(2):447-467. PMC: 7375036. DOI: 10.1007/s00018-020-03600-0. View

20.

Nystedt J, Anderson H, Tikkanen J, Pietila M, Hirvonen T, Takalo R . Cell surface structures influence lung clearance rate of systemically infused mesenchymal stromal cells. Stem Cells. 2012; 31(2):317-26. DOI: 10.1002/stem.1271. View