High-Throughput Identification of MiRNA-Target Interactions in Melanoma Using MiR-CATCHv2.0

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2021 Mar 11

PMID 33704736

Authors

Andrea Marranci

Romina DAurizio

Milena Rizzo

Catherine M Greene

Laura Poliseno

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) can regulate the expression of potentially every transcript in the cell, and the definition of miRNA-target interactions is crucial to understand their role in all biological processes. However, the identification of the miRNAs that target a specific mRNA remains a challenge. Here, we describe an innovative method called miR-CATCHv2.0 for the high-throughput identification of the miRNA species bound to an RNA of interest. We also describe how this method can overcome the limitations of the current computational and experimental methods available in this field.

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