Identification of Suitable Reference Genes for Mesenchymal Stem Cells from Menstrual Blood of Women with Endometriosis

Overview

Journal Sci Rep

Specialty Science

Date 2021 Mar 9

PMID 33686153

Citations 6

Authors

Victoria S Zucherato

Leticia B C Penariol

Lilian E C M Silva

Cristiana C Padovan

Omero B Poli-Neto

Julio C Rosa-E-Silva

Rui A Ferriani

Juliana Meola

Affiliations

Soon will be listed here.

Abstract

It has been suggested that menstrual blood-derived mesenchymal stem/stromal cells (MenMSCs) are associated with the etiopathogenesis of endometriosis and considerable effort has been invested in searching for target genes and deciphering associated molecular pathways. However, reference gene stability for proper reproducible normalization in the analyses of the expression data validation is still unexplored in this experimental context. Therefore, in this exploratory study, we used stringent case and control selection criteria and collected menstrual blood from women with a laparoscopic diagnosis of advanced endometriosis and from fertile women without endometriosis. We tested for the first time the stability of 32 candidate reference genes to achieve increased accuracy and reliable results in the quantification of gene expression and direct future experiments using reverse transcription-quantitative PCR (RT-qPCR) in MenMSCs for endometriosis studies. Using the RefFinder web tool, we recommend the EIF2B1 and POP4 reference genes for the normalization of RT-qPCR data in study designs similar to ours. Furthermore, we suggest avoiding the commonly used GAPDH and ACTB reference genes as they are unstable. This high-visibility study is capable of directing different experimental designs as MenMSCs are derived from a minimally invasive tissue source with multifunctional roles in regenerative medicine.

Citing Articles

Selection of reference miRNAs for RT-qPCR assays in endometriosis menstrual blood-derived mesenchymal stem cells.

Hacimoto S, Cressoni A, Silva L, Padovan C, Ferriani R, Rosa-E-Silva J PLoS One. 2024; 19(7):e0306657.

PMID: 39078824 PMC: 11288454. DOI: 10.1371/journal.pone.0306657.

Stable Housekeeping Genes in Bone Marrow, Adipose Tissue, and Amniotic Membrane-Derived Mesenchymal Stromal Cells for Orthopedic Regenerative Medicine Approaches.

Ragni E, Piccolo S, Papait A, De Luca P, Taiana M, Grieco G Int J Mol Sci. 2024; 25(3).

PMID: 38338737 PMC: 10855448. DOI: 10.3390/ijms25031461.

Downregulation of : Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells?.

Cressoni A, Penariol L, Padovan C, Orellana M, Rosa-E-Silva J, Poli-Neto O Int J Mol Sci. 2023; 24(6).

PMID: 36983035 PMC: 10057010. DOI: 10.3390/ijms24065963.

The Emerging Role of Menstrual-Blood-Derived Stem Cells in Endometriosis.

Cordeiro M, Carvalhos C, Figueiredo-Dias M Biomedicines. 2023; 11(1).

PMID: 36672546 PMC: 9856091. DOI: 10.3390/biomedicines11010039.

What Do the Transcriptome and Proteome of Menstrual Blood-Derived Mesenchymal Stem Cells Tell Us about Endometriosis?.

Penariol L, Thome C, Tozetti P, Paier C, Buono F, Peronni K Int J Mol Sci. 2022; 23(19).

PMID: 36232817 PMC: 9570451. DOI: 10.3390/ijms231911515.

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