QBase Relative Quantification Framework and Software for Management and Automated Analysis of Real-time Quantitative PCR Data

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2007 Feb 13

PMID 17291332

Citations 1824

Authors

Jan Hellemans

Geert Mortier

Anne De Paepe

Frank Speleman

Jo Vandesompele

Affiliations

Soon will be listed here.

Abstract

Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data.

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