Preparing Single-cell DNA Library Using Nextera for Detection of CNV

Overview

Journal Bio Protoc

Specialty Biology

Date 2021 Mar 3

PMID 33654981

Citations 2

Authors

Larry Xi

Patrick Leong

Aleksandar Mihajlovic

Affiliations

Soon will be listed here.

Abstract

Single-cell DNA sequencing is a powerful tool to evaluate the state of heterogeneity of heterogeneous tissues like cancer in a quantitative manner that bulk sequencing can never achieve. DOP-PCR (Degenerate Oligonucleotide-Primed Polymerase Chain Reaction), MDA (Multiple Displacement Amplification), MALBAC (Multiple Annealing and Looping-Based Amplification Cycles), LIANTI (Linear Amplification via Transposon Insertion) and TnBC (Transposon Barcoded) have been the primary choices to prepare single-cell libraries. TnBC library prep method is a simple and versatile methodology, to detect copy number variations or to obtain the absolute copy numbers of genes per cell.

Citing Articles

Barcoded multiple displacement amplification for high coverage sequencing in spatial genomics.

Kim J, Kim S, Yeom H, Song S, Shin K, Bae S Nat Commun. 2023; 14(1):5261.

PMID: 37644058 PMC: 10465490. DOI: 10.1038/s41467-023-41019-w.

Reconstructing and counting genomic fragments through tagmentation-based haploid phasing.

Leong P, Mihajlovic A, Bogdanovic N, Breberina L, Xi L Sci Rep. 2021; 11(1):18907.

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