Development and Application of a Real-time RT-PCR Assay to Rapidly Detect H2 Subtype Avian Influenza A Viruses

Overview

Journal J Vet Diagn Invest

Specialty Veterinary Medicine

Date 2021 Feb 23

PMID 33618630

Citations 3

Authors

Yixin Xiao

Fan Yang

Fumin Liu

Hangping Yao

Nanping Wu

Haibo Wu

Affiliations

Soon will be listed here.

Abstract

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

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References

Hai-bo W, Chao-tan G, Ru-feng L, Li-Hua X, En-kang W, Jin-biao Y . Characterization of a highly pathogenic H5N1 avian influenza virus isolated from ducks in Eastern China in 2011. Arch Virol. 2012; 157(6):1131-6. DOI: 10.1007/s00705-012-1259-1. View

Wu H, Peng X, Peng X, Cheng L, Wu N . Molecular characterization of novel reassortant H6N2 subtype avian influenza viruses isolated from poultry in Eastern China, in 2014. Infect Genet Evol. 2015; 36:41-45. DOI: 10.1016/j.meegid.2015.08.043. View

Elizalde M, Aguero M, Buitrago D, Yuste M, Arias M, Munoz M . Rapid molecular haemagglutinin subtyping of avian influenza isolates by specific real-time RT-PCR tests. J Virol Methods. 2013; 196:71-81. DOI: 10.1016/j.jviromet.2013.10.031. View

Wu H, Peng X, Peng X, Cheng L, Lu X, Jin C . Genetic and molecular characterization of H9N2 and H5 avian influenza viruses from live poultry markets in Zhejiang Province, eastern China. Sci Rep. 2015; 5:17508. PMC: 4667249. DOI: 10.1038/srep17508. View

Wu H, Peng X, Peng X, Cheng L, Wu N . Genetic and molecular characterization of a novel reassortant H2N8 subtype avian influenza virus isolated from a domestic duck in Zhejiang Province in China. Virus Genes. 2016; 52(6):863-866. DOI: 10.1007/s11262-016-1368-0. View

Palese P, Wang T . Why do influenza virus subtypes die out? A hypothesis. mBio. 2011; 2(5). PMC: 3163940. DOI: 10.1128/mBio.00150-11. View

Liu M, He S, Walker D, Zhou N, Perez D, Mo B . The influenza virus gene pool in a poultry market in South central china. Virology. 2003; 305(2):267-75. DOI: 10.1006/viro.2002.1762. View

McGinnis J, Laplante J, Shudt M, St George K . Next generation sequencing for whole genome analysis and surveillance of influenza A viruses. J Clin Virol. 2016; 79:44-50. DOI: 10.1016/j.jcv.2016.03.005. View

He P, Li Y, Luo J, Wang H, Yan Y, Chen Z . Development and Application of A Duplex Real-Time RT-PCR Assay for Simultaneous Detection of Coxsackievirus A6 and Coxsackievirus A10. Ann Clin Lab Sci. 2017; 47(2):178-183. View

10.

Ueda M, Maeda A, Nakagawa N, Kase T, Kubota R, Takakura H . Application of subtype-specific monoclonal antibodies for rapid detection and identification of influenza A and B viruses. J Clin Microbiol. 1998; 36(2):340-4. PMC: 104539. DOI: 10.1128/JCM.36.2.340-344.1998. View

11.

Kuo R, Yang S, Liu Y, Chen L, Mok C, Kuo S . Influenza A/B virus detection and influenza A virus subtyping with emphasis on the novel H7N9 virus by using multiplex real-time RT-PCR. J Virol Methods. 2014; 208:41-6. DOI: 10.1016/j.jviromet.2014.07.020. View

12.

Nabel G, Wei C, Ledgerwood J . Vaccinate for the next H2N2 pandemic now. Nature. 2011; 471(7337):157-8. DOI: 10.1038/471157a. View

13.

Killian M, Zhang Y, Panigrahy B, Trampel D, Yoon K . Identification and characterization of H2N3 avian influenza virus from backyard poultry and comparison with novel H2N3 swine influenza virus. Avian Dis. 2012; 55(4):611-9. DOI: 10.1637/9749-040111-Reg.1. View

14.

Reneer Z, Ross T . H2 influenza viruses: designing vaccines against future H2 pandemics. Biochem Soc Trans. 2019; 47(1):251-264. DOI: 10.1042/BST20180602. View

15.

Santiago G, Vazquez J, Courtney S, Matias K, Andersen L, Colon C . Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses. Nat Commun. 2018; 9(1):1391. PMC: 5895813. DOI: 10.1038/s41467-018-03772-1. View

16.

Simonsen L, Clarke M, Schonberger L, Arden N, Cox N, Fukuda K . Pandemic versus epidemic influenza mortality: a pattern of changing age distribution. J Infect Dis. 1998; 178(1):53-60. DOI: 10.1086/515616. View

17.

Fournie G, Hog E, Barnett T, Pfeiffer D, Mangtani P . A Systematic Review and Meta-Analysis of Practices Exposing Humans to Avian Influenza Viruses, Their Prevalence, and Rationale. Am J Trop Med Hyg. 2017; 97(2):376-388. PMC: 5544094. DOI: 10.4269/ajtmh.17-0014. View

18.

Komissarov A, Fadeev A, Kosheleva A, Sintsova K, Grudinin M . Development of a realtime RT-PCR assay for the rapid detection of influenza A(H2) viruses. Mol Cell Probes. 2017; 35:57-63. PMC: 7126497. DOI: 10.1016/j.mcp.2017.06.005. View

19.

Peng X, Wu H, Jin C, Yao H, Lu X, Cheng L . Sequence and phylogenetic analysis of H2N7 avian influenza viruses isolated from domestic ducks in Zhejiang Province, Eastern China, 2013. Virus Genes. 2014; 48(2):391-6. DOI: 10.1007/s11262-014-1033-4. View

20.

Kishida N, Sakoda Y, Shiromoto M, Bai G, Isoda N, Takada A . H2N5 influenza virus isolates from terns in Australia: genetic reassortants between those of the Eurasian and American lineages. Virus Genes. 2008; 37(1):16-21. DOI: 10.1007/s11262-008-0235-z. View