Analysis of Gene Expression Using LacZ Reporter Mouse Lines

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2021 Feb 19

PMID 33606204

Authors

Michael Simon Kramer

Robert Feil

Hannes Schmidt

Affiliations

Soon will be listed here.

Abstract

Reporter mice transgenically expressing the bacterial (E. coli) lacZ gene encoding β-galactosidase (β-gal, EC 3.2.1.23) are a versatile and extensively used tool to study gene expression and cell lineage patterns. Enzymatic activity of the β-gal reporter can be effectively visualized at cellular resolution either histochemically using 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) or by immunofluorescent detection using a β-gal-specific antibody. Here, we summarize protocols for the localization of β-gal expressing cells in whole embryos or organs as well as in histological tissue sections of lacZ reporter mice and discuss their limitations and common pitfalls.

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