Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2021 Feb 19

PMID 33604842

Citations 1

Authors

Alberto Maria Luciano

Rodrigo Garcia Barros

Ana Caroline Silva Soares

Jose Buratini

Valentina Lodde

Federica Franciosi

Affiliations

Soon will be listed here.

Abstract

The mammalian ovary is a large source of oocytes organized into follicles at various stages of folliculogenesis. However, only a limited number of them can be used for in vitro embryo production (IVEP), while most have yet to complete growth and development to attain full meiotic and embryonic developmental competence. While the in vitro growth of primordial follicles in the ovarian cortex has the potential to produce mature oocytes, it is still at an experimental stage. The population of early antral follicles (EAFs), instead, may represent a reserve of oocytes close to completing the growth phase, which might be more easily exploited in vitro and could increase the number of female gametes dedicated to IVEP.Here we present in vitro culture strategies that have been developed utilizing physiological parameters to support the specific needs of oocytes at distinct stages of differentiation, in order to expand the source of female gametes for IVEP by maximizing the attainment of fertilizable oocytes. Furthermore, these culture systems provide powerful tools to dissect the molecular processes that direct the final differentiation of the mammalian oocyte.

Citing Articles

Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model.

Dey P, Monferini N, Donadini L, Lodde V, Franciosi F, Luciano A Methods Mol Biol. 2024; 2770:171-182.

PMID: 38351454 DOI: 10.1007/978-1-0716-3698-5_13.

References

Conti M, Franciosi F . Acquisition of oocyte competence to develop as an embryo: integrated nuclear and cytoplasmic events. Hum Reprod Update. 2018; 24(3):245-266. PMC: 5907346. DOI: 10.1093/humupd/dmx040. View

Telfer E . Future developments: In vitro growth (IVG) of human ovarian follicles. Acta Obstet Gynecol Scand. 2019; 98(5):653-658. DOI: 10.1111/aogs.13592. View

Luciano A, Franciosi F, Modina S, Lodde V . Gap junction-mediated communications regulate chromatin remodeling during bovine oocyte growth and differentiation through cAMP-dependent mechanism(s). Biol Reprod. 2011; 85(6):1252-9. DOI: 10.1095/biolreprod.111.092858. View

Fair T, Hyttel P, Greve T . Bovine oocyte diameter in relation to maturational competence and transcriptional activity. Mol Reprod Dev. 1995; 42(4):437-42. DOI: 10.1002/mrd.1080420410. View

Lodde V, Modina S, Galbusera C, Franciosi F, Luciano A . Large-scale chromatin remodeling in germinal vesicle bovine oocytes: interplay with gap junction functionality and developmental competence. Mol Reprod Dev. 2006; 74(6):740-9. DOI: 10.1002/mrd.20639. View

Lodde V, Modina S, Maddox-Hyttel P, Franciosi F, Lauria A, Luciano A . Oocyte morphology and transcriptional silencing in relation to chromatin remodeling during the final phases of bovine oocyte growth. Mol Reprod Dev. 2007; 75(5):915-24. DOI: 10.1002/mrd.20824. View

Luciano A, Franciosi F, Dieci C, Lodde V . Changes in large-scale chromatin structure and function during oogenesis: a journey in company with follicular cells. Anim Reprod Sci. 2014; 149(1-2):3-10. DOI: 10.1016/j.anireprosci.2014.06.026. View

Dieci C, Lodde V, Labreque R, Dufort I, Tessaro I, Sirard M . Differences in cumulus cell gene expression indicate the benefit of a pre-maturation step to improve in-vitro bovine embryo production. Mol Hum Reprod. 2016; 22(12):882-897. DOI: 10.1093/molehr/gaw055. View

Lodde V, Modina S, Franciosi F, Zuccari E, Tessaro I, Luciano A . Localization of DNA methyltransferase-1 during oocyte differentiation, in vitro maturation and early embryonic development in cow. Eur J Histochem. 2011; 53(4):e24. PMC: 3167337. DOI: 10.4081/ejh.2009.e24. View

10.

Labrecque R, Lodde V, Dieci C, Tessaro I, Luciano A, Sirard M . Chromatin remodelling and histone m RNA accumulation in bovine germinal vesicle oocytes. Mol Reprod Dev. 2015; 82(6):450-62. DOI: 10.1002/mrd.22494. View

11.

Lodde V, Luciano A, Franciosi F, Labrecque R, Sirard M . Accumulation of Chromatin Remodelling Enzyme and Histone Transcripts in Bovine Oocytes. Results Probl Cell Differ. 2017; 63:223-255. DOI: 10.1007/978-3-319-60855-6_11. View

12.

Lodde V, Franciosi F, Tessaro I, Modina S, Luciano A . Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte. J Assist Reprod Genet. 2013; 30(9):1219-26. PMC: 3800535. DOI: 10.1007/s10815-013-0061-7. View

13.

Franciosi F, Coticchio G, Lodde V, Tessaro I, Modina S, Fadini R . Natriuretic peptide precursor C delays meiotic resumption and sustains gap junction-mediated communication in bovine cumulus-enclosed oocytes. Biol Reprod. 2014; 91(3):61. DOI: 10.1095/biolreprod.114.118869. View

14.

Soares A, Marques K, Braganca L, Lodde V, Luciano A, Buratini J . Synchronization of germinal vesicle maturity improves efficacy of in vitro embryo production in Holstein cows. Theriogenology. 2020; 154:53-58. DOI: 10.1016/j.theriogenology.2020.05.030. View

15.

Soares A, Sakoda J, Gama I, Bayeux B, Lodde V, Luciano A . Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds. Theriogenology. 2019; 141:146-152. DOI: 10.1016/j.theriogenology.2019.09.020. View

16.

Zhang M, Su Y, Sugiura K, Xia G, Eppig J . Granulosa cell ligand NPPC and its receptor NPR2 maintain meiotic arrest in mouse oocytes. Science. 2010; 330(6002):366-9. PMC: 3056542. DOI: 10.1126/science.1193573. View

17.

Dieci C, Lodde V, Franciosi F, Lagutina I, Tessaro I, Modina S . The effect of cilostamide on gap junction communication dynamics, chromatin remodeling, and competence acquisition in pig oocytes following parthenogenetic activation and nuclear transfer. Biol Reprod. 2013; 89(3):68. DOI: 10.1095/biolreprod.113.110577. View

18.

Shu Y, Zeng H, Ren Z, Zhuang G, Liang X, Shen H . Effects of cilostamide and forskolin on the meiotic resumption and embryonic development of immature human oocytes. Hum Reprod. 2008; 23(3):504-13. DOI: 10.1093/humrep/dem344. View

19.

Alam M, Lee J, Miyano T . Inhibition of PDE3A sustains meiotic arrest and gap junction of bovine growing oocytes in in vitro growth culture. Theriogenology. 2018; 118:110-118. DOI: 10.1016/j.theriogenology.2018.05.028. View

20.

Tsafriri A, Chun S, Zhang R, Hsueh A, Conti M . Oocyte maturation involves compartmentalization and opposing changes of cAMP levels in follicular somatic and germ cells: studies using selective phosphodiesterase inhibitors. Dev Biol. 1996; 178(2):393-402. DOI: 10.1006/dbio.1996.0226. View