The Durum Wheat Annexin, TdAnn6, Improves Salt and Osmotic Stress Tolerance in Arabidopsis Via Modulation of Antioxidant Machinery

Overview

Journal Protoplasma

Publisher Springer

Specialty Biology

Date 2021 Feb 17

PMID 33594480

Citations 6

Authors

Marwa Harbaoui

Walid Ben Romdhane

Anis Ben Hsouna

Faical Brini

Rania Ben Saad

Affiliations

Soon will be listed here.

Abstract

TdAnn6 is a gene encoding an annexin protein in durum wheat (Triticum durum). The function of TdAnn6 in plant response to stress is not yet clearly understood. Here, we isolated TdAnn6 and characterized it in genetically modified Arabidopsis thaliana. Expressing TdAnn6 in Arabidopsis coincided with an improvement in stress tolerance at germination and seedling stages. In addition, TdAnn6-expressing seedling antioxidant activities were improved with lower level of malondialdehyde, and enhanced transcript levels of six stress-related genes during salt/osmotic stresses. Under greenhouse conditions, the TdAnn6 plants exhibited increased tolerance to salt or drought stress. To deepen our understanding of TdAnn6 function, we isolated a 1515-bp genomic fragment upstream of its coding sequence, designated as PrTdAnn6. The PrTdAnn6 promoter was fused to the β-glucuronidase reporter gene and transferred to Arabidopsis. By histochemical GUS staining, GUS activity was detected in the roots, leaves, and floral organs, but no activity was detected in the seeds. Furthermore, we noticed a high stimulation of promoter activity when A. thaliana seedlings were exposed to NaCl, mannitol, ABA, GA, and cold conditions. This cross-talk between tissue-specific expression and exogenous stress stimulation may provide additional layers of regulation for salt and osmotic stress responses in crops.

Citing Articles

The Evolution, Expression Patterns, and Domestication Selection Analysis of the Annexin Gene Family in the Barley Pan-Genome.

Chen L, Chen K, Xi X, Du X, Zou X, Ma Y Int J Mol Sci. 2024; 25(7).

PMID: 38612691 PMC: 11011394. DOI: 10.3390/ijms25073883.

Genome-Wide Identification of the Gene Family and Functional Validation of and under Abiotic Stress.

Luo J, Li M, Ju J, Hai H, Wei W, Ling P Int J Mol Sci. 2024; 25(3).

PMID: 38339155 PMC: 10855742. DOI: 10.3390/ijms25031877.

Rice OsANN9 Enhances Drought Tolerance through Modulating ROS Scavenging Systems.

Jia Y, Gu X, Chai J, Yao X, Cheng S, Liu L Int J Mol Sci. 2023; 24(24).

PMID: 38139326 PMC: 10743917. DOI: 10.3390/ijms242417495.

Genome-wide analysis of annexin gene family in Schrenkiella parvula and Eutrema salsugineum suggests their roles in salt stress response.

Moinoddini F, Kakhki A, Bagheri A, Jalilian A PLoS One. 2023; 18(1):e0280246.

PMID: 36652493 PMC: 9847905. DOI: 10.1371/journal.pone.0280246.

Bio-Stimulating Effect of Natural Polysaccharides from on Durum Wheat Seedlings: Improved Plant Growth, Salt Stress Tolerance by Modulating Biochemical Responses and Ion Homeostasis.

Bouteraa M, Mishra A, Ben Romdhane W, Ben Hsouna A, Siddique K, Ben Saad R Plants (Basel). 2022; 11(15).

PMID: 35956469 PMC: 9370194. DOI: 10.3390/plants11151991.

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