Involvement of Three Annexin Genes in the Ripening of Strawberry Fruit Regulated by Phytohormone and Calcium Signal Transduction

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2016 Jan 4

PMID 26724928

Citations 15

Authors

Jingxin Chen

Linchun Mao

Hongbo Mi

Wenjing Lu

Tiejin Ying

Zisheng Luo

Affiliations

Soon will be listed here.

Abstract

Three annexin genes may be involved in the ripening progress of strawberry fruit. Phytohormones and calcium regulate the expressions of three annexin genes during strawberry fruit ripening. Plant annexins are multi-functional membrane- and Ca(2+)-binding proteins that are involved in various developmental progresses and stress responses. Three annexins FaAnn5a, FaAnn5b and FaAnn8 cDNA obtained from strawberry fruit encode amino acid sequences of approximately 35 kDa containing four annexin repeats, Ca(2+)-binding site, GTP-binding motif, peroxidase residue, and conserved amino acid residues of tryptophan, arginine and cysteine. During fruit development, the transcript levels of FaAnn5a and FaAnn5b increased while FaAnn5b declined after 3/4R stage. The expression patterns of annexins suggested their potential roles in strawberry fruit development and ripening. Expressions of annexin genes were also highly correlated with hormone levels. In addition, exogenous abscisic acid (ABA) enhanced the expressions of FaAnn5a and FaAnn8 while exogenous auxin (IAA) retarded it. However, both ABA and IAA promoted the transcript levels of FaAnn5b, indicating the independent regulation of annexins in fruit likely due to multi-functions of their large family. The responses of annexin genes to exogenous ABA and IAA inhibitors verified the involvement of annexins in plant hormone signaling. Besides, calcium restrained the expressions of FaAnn5s (FaAnn5a and FaAnn5b) but promoted the expression of FaAnn8. Effects of calcium and ethylene glycol tetraacetic acid (EGTA) on the transcript levels of annexins confirmed that calcium likely mediated hormone signal transduction pathways, which helped to elucidate the mechanism of calcium in fruit ripening. Therefore, FaAnn5s and FaAnn8 might be involved in plant hormones' regulation in the development and ripening of strawberry fruit through calcium signaling in the downstream.

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References

Bai S, Willard B, Chapin L, Kinter M, Francis D, Stead A . Proteomic analysis of pollination-induced corolla senescence in petunia. J Exp Bot. 2010; 61(4):1089-109. PMC: 2826652. DOI: 10.1093/jxb/erp373. View

Gerke V, Creutz C, Moss S . Annexins: linking Ca2+ signalling to membrane dynamics. Nat Rev Mol Cell Biol. 2005; 6(6):449-61. DOI: 10.1038/nrm1661. View

Konopka-Postupolska D, Clark G, Goch G, Debski J, Floras K, Cantero A . The role of annexin 1 in drought stress in Arabidopsis. Plant Physiol. 2009; 150(3):1394-410. PMC: 2705051. DOI: 10.1104/pp.109.135228. View

Gorecka K, Konopka-Postupolska D, Hennig J, Buchet R, Pikula S . Peroxidase activity of annexin 1 from Arabidopsis thaliana. Biochem Biophys Res Commun. 2005; 336(3):868-75. DOI: 10.1016/j.bbrc.2005.08.181. View

Vandeputte O, Oukouomi Lowe Y, Burssens S, VAN Raemdonck D, Hutin D, Boniver D . The tobacco Ntann12 gene, encoding an annexin, is induced upon Rhodoccocus fascians infection and during leafy gall development. Mol Plant Pathol. 2010; 8(2):185-94. DOI: 10.1111/j.1364-3703.2007.00385.x. View

Clark G, Sessions A, Eastburn D, Roux S . Differential expression of members of the annexin multigene family in Arabidopsis. Plant Physiol. 2001; 126(3):1072-84. PMC: 116464. DOI: 10.1104/pp.126.3.1072. View

Symons G, Chua Y, Ross J, Quittenden L, Davies N, Reid J . Hormonal changes during non-climacteric ripening in strawberry. J Exp Bot. 2012; 63(13):4741-50. PMC: 3428006. DOI: 10.1093/jxb/ers147. View

Wilkinson J, Lanahan M, Conner T, Klee H . Identification of mRNAs with enhanced expression in ripening strawberry fruit using polymerase chain reaction differential display. Plant Mol Biol. 1995; 27(6):1097-108. DOI: 10.1007/BF00020883. View

Proust J, Houlne G, Schantz M, Schantz R . Characterization and gene expression of an annexin during fruit development in Capsicum annuum. FEBS Lett. 1996; 383(3):208-12. DOI: 10.1016/0014-5793(96)00252-9. View

10.

Reddy V, Reddy A . Proteomics of calcium-signaling components in plants. Phytochemistry. 2004; 65(12):1745-76. DOI: 10.1016/j.phytochem.2004.04.033. View

11.

Talukdar T, Gorecka K, de Carvalho-Niebel F, Downie J, Cullimore J, Pikula S . Annexins - calcium- and membrane-binding proteins in the plant kingdom: potential role in nodulation and mycorrhization in Medicago truncatula. Acta Biochim Pol. 2009; 56(2):199-210. View

12.

Mori I, Schroeder J . Reactive oxygen species activation of plant Ca2+ channels. A signaling mechanism in polar growth, hormone transduction, stress signaling, and hypothetically mechanotransduction. Plant Physiol. 2004; 135(2):702-8. PMC: 514107. DOI: 10.1104/pp.104.042069. View

13.

Clark G, Morgan R, Fernandez M, Roux S . Evolutionary adaptation of plant annexins has diversified their molecular structures, interactions and functional roles. New Phytol. 2012; 196(3):695-712. DOI: 10.1111/j.1469-8137.2012.04308.x. View

14.

Bianco L, Lopez L, Scalone A, Di Carli M, Desiderio A, Benvenuto E . Strawberry proteome characterization and its regulation during fruit ripening and in different genotypes. J Proteomics. 2009; 72(4):586-607. DOI: 10.1016/j.jprot.2008.11.019. View

15.

Baucher M, Oukouomi Lowe Y, Vandeputte O, Mukoko Bopopi J, Moussawi J, Vermeersch M . Ntann12 annexin expression is induced by auxin in tobacco roots. J Exp Bot. 2011; 62(11):4055-65. PMC: 3134359. DOI: 10.1093/jxb/err112. View

16.

Zhang F, Li S, Yang S, Wang L, Guo W . Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton. Plant Mol Biol. 2014; 87(1-2):47-67. DOI: 10.1007/s11103-014-0260-3. View

17.

Delmer D, Potikha T . Structures and functions of annexins in plants. Cell Mol Life Sci. 1997; 53(6):546-53. PMC: 11147188. DOI: 10.1007/s000180050070. View

18.

Kovacs I, Ayaydin F, Oberschall A, Ipacs I, Bottka S, Pongor S . Immunolocalization of a novel annexin-like protein encoded by a stress and abscisic acid responsive gene in alfalfa. Plant J. 1998; 15(2):185-97. DOI: 10.1046/j.1365-313x.1998.00194.x. View

19.

Laohavisit A, Davies J . Annexins. New Phytol. 2010; 189(1):40-53. DOI: 10.1111/j.1469-8137.2010.03533.x. View

20.

Tang W, He Y, Tu L, Wang M, Li Y, Ruan Y . Down-regulating annexin gene GhAnn2 inhibits cotton fiber elongation and decreases Ca2+ influx at the cell apex. Plant Mol Biol. 2014; 85(6):613-25. DOI: 10.1007/s11103-014-0208-7. View