Conceptus Metabolomic Profiling Reveals Stage-specific Phenotypes Leading Up to Pregnancy Recognition in Cattle†

Overview

Journal Biol Reprod

Publisher Oxford University Press

Specialty Reproductive Medicine

Date 2021 Feb 16

PMID 33590828

Citations 4

Authors

Constantine A Simintiras

Jose M Sanchez

Michael McDonald

Elena OCallaghan

Ahmed A Aburima

Patrick Lonergan

Affiliations

Soon will be listed here.

Abstract

Reproductive efficiency in livestock is a major driver of sustainable food production. The poorly understood process of ruminant conceptus elongation (a) prerequisites maternal pregnancy recognition, (b) is essential to successful pregnancy establishment, and (c) coincides with a period of significant conceptus mortality. Conceptuses at five key developmental stages between Days 8-16 were recovered and cultured in vitro for 6 h prior to conditioned media analysis by untargeted ultrahigh-performance liquid chromatography tandem mass spectroscopy. This global temporal biochemical interrogation of the ex situ bovine conceptus unearths two antithetical stage-specific metabolic phenotypes during tubular (metabolically retentive) vs. filamentous (secretory) development. Moreover, the retentive conceptus phenotype on Day 14 coincides with an established period of elevated metabolic density in the uterine fluid of heifers with high systemic progesterone-a model of accelerated conceptus elongation. These data, combined, suggest a metabolic mechanism underpinning conceptus elongation, thereby enhancing our understanding of the biochemical reciprocity of maternal-conceptus communication, prior to maternal pregnancy recognition.

Citing Articles

The Impact of Uterus-Derived Prostaglandins on the Composition of Uterine Fluid During the Period of Conceptus Elongation in Dairy Heifers.

Zhang B, Han Y, Wang S, Cheng M, Yan L, Zhou D Int J Mol Sci. 2025; 26(5).

PMID: 40076420 PMC: 11899274. DOI: 10.3390/ijms26051792.

PPARG is dispensable for bovine embryo development up to tubular stages†.

Perez-Gomez A, Gonzalez-Brusi L, Flores-Borobia I, Martinez De Los Reyes N, Toledano-Diaz A, Lopez-Sebastian A Biol Reprod. 2024; 111(3):557-566.

PMID: 38832705 PMC: 11402522. DOI: 10.1093/biolre/ioae083.

Biochemical alterations in the follicular fluid of bovine peri-ovulatory follicles and association with final oocyte maturation.

Alrabiah N, Simintiras C, Evans A, Lonergan P, Fair T Reprod Fertil. 2022; .

PMID: 36547396 PMC: 9874974. DOI: 10.1530/RAF-22-0090.

Uterine lumen fluid is metabolically semi-autonomous.

Simintiras C, Drum J, Liu H, Ortega M, Spencer T Commun Biol. 2022; 5(1):191.

PMID: 35233029 PMC: 8888695. DOI: 10.1038/s42003-022-03134-0.

Capture and metabolomic analysis of the human endometrial epithelial organoid secretome.

Simintiras C, Dhakal P, Ranjit C, Fitzgerald H, Balboula A, Spencer T Proc Natl Acad Sci U S A. 2021; 118(15).

PMID: 33876774 PMC: 8053979. DOI: 10.1073/pnas.2026804118.

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