Liquid Chromatin Hi-C Characterizes Compartment-dependent Chromatin Interaction Dynamics

Overview

Journal Nat Genet

Specialty Genetics

Date 2021 Feb 12

PMID 33574602

Citations 68

Authors

Houda Belaghzal

Tyler Borrman

Andrew D Stephens

Denis L Lafontaine

Sergey V Venev

Zhiping Weng

John F Marko

Job Dekker

Affiliations

Soon will be listed here.

Abstract

Nuclear compartmentalization of active and inactive chromatin is thought to occur through microphase separation mediated by interactions between loci of similar type. The nature and dynamics of these interactions are not known. We developed liquid chromatin Hi-C to map the stability of associations between loci. Before fixation and Hi-C, chromosomes are fragmented, which removes strong polymeric constraint, enabling detection of intrinsic locus-locus interaction stabilities. Compartmentalization is stable when fragments are larger than 10-25 kb. Fragmentation of chromatin into pieces smaller than 6 kb leads to gradual loss of genome organization. Lamin-associated domains are most stable, whereas interactions for speckle- and polycomb-associated loci are more dynamic. Cohesin-mediated loops dissolve after fragmentation. Liquid chromatin Hi-C provides a genome-wide view of chromosome interaction dynamics.

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