Arginine Methylation Regulates Ribosome CAR Function

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Feb 12

PMID 33572867

Citations 3

Authors

Kristen Scopino

Carol Dalgarno

Clara Nachmanoff

Daniel Krizanc

Kelly M Thayer

Michael P Weir

Affiliations

Soon will be listed here.

Abstract

The ribosome CAR interaction surface is hypothesized to provide a layer of translation regulation through hydrogen-bonding to the +1 mRNA codon that is next to enter the ribosome A site during translocation. The CAR surface consists of three residues, 16S/18S rRNA C1054, A1196 ( 16S numbering), and R146 of yeast ribosomal protein Rps3. R146 can be methylated by the Sfm1 methyltransferase which is downregulated in stressed cells. Through molecular dynamics analysis, we show here that methylation of R146 compromises the integrity of CAR by reducing the cation-pi stacking of the R146 guanidinium group with A1196, leading to reduced CAR hydrogen-bonding with the +1 codon. We propose that ribosomes assembled under stressed conditions have unmethylated R146, resulting in elevated CAR/+1 codon interactions, which tunes translation levels in response to the altered cellular context.

Citing Articles

GNN Codon Adjacency Tunes Protein Translation.

Sun J, Hwang P, Sakkas E, Zhou Y, Perez L, Dave I Int J Mol Sci. 2024; 25(11).

PMID: 38892101 PMC: 11172435. DOI: 10.3390/ijms25115914.

CNNArginineMe: A CNN structure for training models for predicting arginine methylation sites based on the One-Hot encoding of peptide sequence.

Zhao J, Jiang H, Zou G, Lin Q, Wang Q, Liu J Front Genet. 2022; 13:1036862.

PMID: 36324513 PMC: 9618650. DOI: 10.3389/fgene.2022.1036862.

The CAR-mRNA Interaction Surface Is a Zipper Extension of the Ribosome A Site.

Dalgarno C, Scopino K, Raval M, Nachmanoff C, Sakkas E, Krizanc D Int J Mol Sci. 2022; 23(3).

PMID: 35163343 PMC: 8835751. DOI: 10.3390/ijms23031417.

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