Two Ecdysteroids Isolated from Micropropagated and the Biological Activity of Plant Material

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Feb 12

PMID 33572129

Citations 5

Authors

Michal P Malinski

Jaromir Budzianowski

Malgorzata Kikowska

Monika Derda

Marcelina M Jaworska

Dariusz T Mlynarczyk

Marta Szukalska

Ewa Florek

Barbara Thiem

Affiliations

Soon will be listed here.

Abstract

Genetically uniform plant material, derived from propagated in vitro, was used for the isolation of 20-hydroxyecdysone and polypodine B and subjected to an evaluation of the antifungal and antiamoebic activity. The activity of 80% aqueous methanolic extracts, their fractions, and isolated ecdysteroids were studied against pathogenic . Additionally, a Microtox acute toxicity assay was performed. It was found that an 80% methanolic fraction of root extract exerts the most potent amoebicidal activity at IC of 0.06 mg/mL at the 3rd day of treatment. Both ecdysteroids show comparable activity at IC of 0.07 mg/mL. The acute toxicity of 80% fractions at similar concentrations is significantly higher than that of 40% fractions. Crude extracts exhibited moderate antifungal activity, with a minimum inhibitory concentration (MIC) within the range of 1.25-2.5 mg/mL. To the best of our knowledge, the present report is the first to show the biological activity of in terms of the antifungal and antiamoebic activities and acute toxicity. It is also the first isolation of the main ecdysteroids from micropropagated, ecdysteroid-rich plant material.

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