Generation of Sex-Reversed Female Clonal Mice Via CRISPR-Cas9-Mediated Y Chromosome Deletion in Male Embryonic Stem Cells

Overview

Journal CRISPR J

Specialty Molecular Biology

Date 2021 Feb 10

PMID 33567216

Authors

Yiren Qin

Bokey Wong

Liangwen Zhong

Fuqiang Geng

Luis F Parada

Duancheng Wen

Affiliations

Soon will be listed here.

Abstract

Mice derived entirely from embryonic stem (ES) cells can be generated through tetraploid complementation. Although XY male ES cell lines are commonly used in this system, occasionally, monosomic XO female mice are produced through spontaneous Y chromosome loss. Here, we describe an efficient method to obtain monosomic XO ES cells by CRISPR-Cas9-mediated deletion of the Y chromosome, allowing generation of female clonal mice by tetraploid complementation. The monosomic XO female mice are viable and able to produce normal male and female offspring. Direct generation of clonal mice in both sexes can significantly accelerate the production of complex genetically modified mouse models.

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