Icariin Attenuates Endothelial-mesenchymal Transition Via H19/miR-148b-3p/ELF5 in Ox-LDL-stimulated HUVECs

Overview

Journal Mol Ther Nucleic Acids

Publisher Cell Press

Date 2021 Jan 29

PMID 33510936

Citations 11

Authors

Shan Liu

Dong-Sheng Xu

Min Li

Yang Zhang

Qi Li

Teng-Teng Li

Li-Qun Ren

Affiliations

Soon will be listed here.

Abstract

Atherosclerosis is the main cause of cardio-cerebrovascular diseases. Endothelial-mesenchymal transition plays an important role in atherosclerosis. Icariin has a protective effect on atherosclerosis; however, the underlying mechanism remains unclear. In this study, we explored the molecular mechanism underlying the protective function of icariin in oxidized low-density lipoprotein-stimulated human umbilical vein endothelial cells. H19, a long non-coding RNA, was identified to be downregulated in the background of the oxidized low-density lipoprotein-induced endothelial-mesenchymal transition in human umbilical vein endothelial cells. Icariin upregulated H19 expression and inhibited the transformation of endothelial cells into interstitial cells. Overexpression of H19 affected endothelial-mesenchymal transition in oxidized low-density lipoprotein-stimulated human umbilical vein endothelial cells, whereas H19 knockdown reversed endothelial protective effects of icariin and reduced human umbilical vein endothelial cell migration. Knockdown of H19 significantly downregulated oxidized low-density lipoprotein-induced E74-like factor 5 and upregulated miR-148b-3p, which was reversed by icariin. Thus, icariin may play a protective role in atherosclerosis, and H19 may be a potential therapeutic target.

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