Broad Specificity of Immune Helminth ScFv Library to Identify Monoclonal Antibodies Targeting Strongyloides

Overview

Journal Sci Rep

Specialty Science

Date 2021 Jan 29

PMID 33510342

Citations 5

Authors

Anizah Rahumatullah

Dinesh Balachandra

Rahmah Noordin

Zamrina Baharudeen

Yee Ying Lim

Yee Siew Choong

Theam Soon Lim

Affiliations

Soon will be listed here.

Abstract

Antibodies have different chemical properties capable of targeting a diverse nature of antigens. Traditionally, immune antibody libraries are perceived to be disease-specific with a skewed repertoire. The complexity during the generation of a combinatorial antibody library allows for a skewed but diverse repertoire to be generated. Strongyloides stercoralis is a parasite that causes strongyloidiasis, a potentially life-threatening disease with a complex diagnosis that impedes effective control and treatment of the disease. This study describes the isolation of monoclonal antibodies against S. stercoralis NIE recombinant protein using an immune antibody phage display library derived from lymphatic filaria-infected individuals. The isolated antibody clones showed both lambda and kappa light chains gene usage, with diverse amino acid distributions. Structural analysis showed that electropositivity and the interface area could determine the binding affinity of the clones with NIE. The successful identification of S. stercoralis antibodies from the filarial immune library highlights the breadth of antibody gene diversification in an immune antibody library that can be applied for closely related infections.

Citing Articles

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Antibody Phage Display.

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Isolation and Production of Human Monoclonal Antibody Proteins against a Excretory-Secretory Recombinant Antigen.

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