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Initial Investigations of a Novel Bioluminescence Method for Imaging Dental Demineralization

Overview
Publisher Wiley
Specialty Dentistry
Date 2021 Jan 28
PMID 33507620
Citations 6
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Abstract

Objectives: In this in vitro study, a bioluminescent marker was investigated for its potential to illuminate the assessment of dental caries and dental erosion, which are significant clinical and public health problems, through its binding of those ions, notably Ca , known to be released during the process of demineralization.

Materials And Methods: The light output from the selected bioluminescent marker was investigated in several experiments, including: (a)contact with a range of Ca ion concentrations; (b) treatment of extracted teeth with solutions of differing pH, followed by application of the bioluminescence marker to assess Ca ion release; and (c) application of the marker to freshly extracted teeth with natural and artificially created caries lesions on occlusal and smooth surfaces to image the Ca ion distribution.

Results: The results of: experiment (a) showed that the light output from the marker increases with increasing Ca concentration and of experiment (b) showed increases in light being observed as increasingly acidic solutions were applied. The results of experiment (c) showed the bioluminescence images of the extracted teeth produced "demineralization maps" of the imaged surfaces.

Conclusions: These results demonstrate the ability of a novel bioluminescence technology to image Ca ions on tooth enamel surfaces which has potential in dental caries and dental erosion applications and provides the scientific basis for the ongoing development of that novel technology.

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Pathophysiology of Demineralization, Part I: Attrition, Erosion, Abfraction, and Noncarious Cervical Lesions.

Eugene Roberts W, Mangum J, Schneider P Curr Osteoporos Rep. 2022; 20(1):90-105.

PMID: 35129809 PMC: 8930910. DOI: 10.1007/s11914-022-00722-1.


Agreement of Bioluminescence Measurements and Visual Assessment in Monitoring Occlusal Surfaces of Permanent Teeth.

Jablonski-Momeni A, Jablonski B, Heinzel-Gutenbrunner M, Korbmacher-Steiner H J Clin Med. 2022; 11(2).

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The Calcivis story - enamel caries activity assessment from technology to practice.

Pitts N, Longbottom C, Christie A, Vernon B, Bailey G Br Dent J. 2021; 231(12):775-780.

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Use of bioluminescence measurements for detection of artificial demineralization adjacent to orthodontic brackets.

Jablonski-Momeni A, Sambale J, Gaerttner L, Nothelfer R, Korbmacher-Steiner H J Orofac Orthop. 2021; 84(4):207-215.

PMID: 34331070 PMC: 10281892. DOI: 10.1007/s00056-021-00341-y.


References
1.
Lussi A, Imwinkelried S, Pitts N, Longbottom C, Reich E . Performance and reproducibility of a laser fluorescence system for detection of occlusal caries in vitro. Caries Res. 1999; 33(4):261-6. DOI: 10.1159/000016527. View

2.
Van de Rijke J . Use of dyes in cariology. Int Dent J. 1991; 41(2):111-6. View

3.
Konikoff B, Lyles G . "In vivo" method for detection of soluble calcium in enamel and its developmental philosophy. J La Dent Assoc. 1974; 32(3):7-13. View

4.
Rawls H, Owen W . Demonstration of dye-uptake as a potential aid in early diagnosis of incipient caries. Caries Res. 1978; 12(2):69-75. DOI: 10.1159/000260317. View

5.
Neuhaus K, Longbottom C, Ellwood R, Lussi A . Novel lesion detection aids. Monogr Oral Sci. 2009; 21:52-62. DOI: 10.1159/000224212. View