Laccase Immobilization on Magnetic Nanoparticles to Improve Stability and Its Potential Application in Bisphenol A Degradation

Overview

Journal Indian J Microbiol

Specialty Microbiology

Date 2021 Jan 28

PMID 33505092

Citations 29

Authors

Sanjay K S Patel

Rahul K Gupta

Sang-Yong Kim

In-Won Kim

Vipin C Kalia

Jung-Kul Lee

Affiliations

Soon will be listed here.

Abstract

In the present study, laccase (Lac) was immobilized through covalent methods on the magnetic nanoparticles. FeO and FeO nanoparticles activated by 3-aminopropyltriethoxysilane followed with glutaraldehyde showed maximum immobilization yields and relative activity up to 81.4 and 84.3% at optimum incubation and pH of 18 h and 5.8, respectively. The maximum Lac loading of 156 mg/g of support was recorded on FeO nanoparticles. A higher optimum pH and temperature of 4.0 and 45 °C were noted for immobilized enzyme compared to values of 3.5 and 40 °C for free form, respectively. Immobilized Lac exhibited better relative activity profiles at various pH and temperature ranges. The immobilized enzyme showed up to 16-fold improvement in the thermal stability, when incubated at 60 °C, and retained up to 82.9% of residual activity after ten cycles of reuses. Immobilized Lac exhibited up to 1.9-fold higher bisphenol A degradation efficiency potential over free enzyme. Previous reports have demonstrated the immobilization of Lac on non-magnetic supports. This study has demonstrated that immobilization of Lac on magnetic nanoparticles is very efficient especially for achieving high loading, better pH and temperature profiles, and thermal- and solvents-stability, high reusability, and higher degradation of bisphenol A.

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References

Patel S, Kumar V, Mardina P, Li J, Lestari R, Kalia V . Methanol production from simulated biogas mixtures by co-immobilized Methylomonas methanica and Methylocella tundrae. Bioresour Technol. 2018; 263:25-32. DOI: 10.1016/j.biortech.2018.04.096. View

Patel S, Jeon M, Gupta R, Jeon Y, Kalia V, Kim S . Hierarchical Macroporous Particles for Efficient Whole-Cell Immobilization: Application in Bioconversion of Greenhouse Gases to Methanol. ACS Appl Mater Interfaces. 2019; 11(21):18968-18977. DOI: 10.1021/acsami.9b03420. View

Patel S, Otari S, Li J, Kim D, Kim S, Cho B . Synthesis of cross-linked protein-metal hybrid nanoflowers and its application in repeated batch decolorization of synthetic dyes. J Hazard Mater. 2018; 347:442-450. DOI: 10.1016/j.jhazmat.2018.01.003. View

Fernandez-Fernandez M, Sanroman M, Moldes D . Recent developments and applications of immobilized laccase. Biotechnol Adv. 2012; 31(8):1808-25. DOI: 10.1016/j.biotechadv.2012.02.013. View

Patel S, Choi S, Kang Y, Lee J . Large-scale aerosol-assisted synthesis of biofriendly Fe₂O₃ yolk-shell particles: a promising support for enzyme immobilization. Nanoscale. 2016; 8(12):6728-38. DOI: 10.1039/c6nr00346j. View

Angural S, Rana M, Sharma A, Warmoota R, Puri N, Gupta N . Combinatorial Biobleaching of Mixedwood Pulp with Lignolytic and Hemicellulolytic Enzymes for Paper Making. Indian J Microbiol. 2020; 60(3):383-387. PMC: 7329959. DOI: 10.1007/s12088-020-00867-6. View

Patel S, Choi S, Kang Y, Lee J . Eco-Friendly Composite of FeO-Reduced Graphene Oxide Particles for Efficient Enzyme Immobilization. ACS Appl Mater Interfaces. 2016; 9(3):2213-2222. DOI: 10.1021/acsami.6b05165. View

Kumar A, Patel S, Mardan B, Pagolu R, Lestari R, Jeong S . Immobilization of Xylanase Using a Protein-Inorganic Hybrid System. J Microbiol Biotechnol. 2018; 28(4):638-644. DOI: 10.4014/jmb.1710.10037. View

Patel S, Kim J, Kalia V, Lee J . Antimicrobial Activity of Amino-Derivatized Cationic Polysaccharides. Indian J Microbiol. 2019; 59(1):96-99. PMC: 6328405. DOI: 10.1007/s12088-018-0764-7. View

10.

Zhang C, You S, Liu Y, Wang C, Yan Q, Qi W . Construction of luffa sponge-based magnetic carbon nanocarriers for laccase immobilization and its application in the removal of bisphenol A. Bioresour Technol. 2020; 305:123085. DOI: 10.1016/j.biortech.2020.123085. View

11.

Kondaveeti S, Patel S, Woo J, Wee J, Kim S, Al-Raoush R . Characterization of Cellobiohydrolases from KMJ820. Indian J Microbiol. 2020; 60(2):160-166. PMC: 7105533. DOI: 10.1007/s12088-019-00843-9. View

12.

Singh G, Kaur K, Puri S, Sharma P . Critical factors affecting laccase-mediated biobleaching of pulp in paper industry. Appl Microbiol Biotechnol. 2014; 99(1):155-64. DOI: 10.1007/s00253-014-6219-0. View

13.

Kumar V, Patel S, Gupta R, Otari S, Gao H, Lee J . Enhanced Saccharification and Fermentation of Rice Straw by Reducing the Concentration of Phenolic Compounds Using an Immobilized Enzyme Cocktail. Biotechnol J. 2019; 14(6):e1800468. DOI: 10.1002/biot.201800468. View

14.

Lee J, Patel S, Sung B, Kalia V . Biomolecules from municipal and food industry wastes: An overview. Bioresour Technol. 2019; 298:122346. DOI: 10.1016/j.biortech.2019.122346. View

15.

Narain Singh D, Sood U, Singh A, Gupta V, Shakarad M, Rawat C . Genome Sequencing Revealed the Biotechnological Potential of an Obligate Thermophile Strain RL Isolated from Hot Water Spring. Indian J Microbiol. 2019; 59(3):351-355. PMC: 6646505. DOI: 10.1007/s12088-019-00809-x. View

16.

Gao H, Li J, Sivakumar D, Kim T, Patel S, Kalia V . NADH oxidase from Lactobacillus reuteri: A versatile enzyme for oxidized cofactor regeneration. Int J Biol Macromol. 2018; 123:629-636. DOI: 10.1016/j.ijbiomac.2018.11.096. View

17.

Kumar A, Kim I, Patel S, Lee J . Synthesis of Protein-Inorganic Nanohybrids with Improved Catalytic Properties Using Co(PO). Indian J Microbiol. 2018; 58(1):100-104. PMC: 5801189. DOI: 10.1007/s12088-017-0700-2. View

18.

Otari S, Patel S, Kalia V, Lee J . One-step hydrothermal synthesis of magnetic rice straw for effective lipase immobilization and its application in esterification reaction. Bioresour Technol. 2020; 302:122887. DOI: 10.1016/j.biortech.2020.122887. View

19.

Patel S, Gupta R, Kumar V, Mardina P, Lestari R, Kalia V . Influence of Metal Ions on the Immobilization of β-Glucosidase Through Protein-Inorganic Hybrids. Indian J Microbiol. 2019; 59(3):370-374. PMC: 6646435. DOI: 10.1007/s12088-019-00796-z. View

20.

Patel S, Gupta R, Kondaveeti S, Otari S, Kumar A, Kalia V . Conversion of biogas to methanol by methanotrophs immobilized on chemically modified chitosan. Bioresour Technol. 2020; 315:123791. DOI: 10.1016/j.biortech.2020.123791. View