Characterization of Carboxylic Acid Reductase from Immobilized Onto Seplite LX120

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2022 Oct 27

PMID 36297953

Authors

Rose Syuhada Basri

Raja Noor Zaliha Raja Abd Rahman

Nor Hafizah Ahmad Kamarudin

Wahhida Latip

Mohd Shukuri Mohamad Ali

Affiliations

Soon will be listed here.

Abstract

A multi-domain oxidoreductase, carboxylic acid reductase (CAR), can catalyze the one-step reduction of carboxylic acid to aldehyde. This study aimed to immobilize bacterial CAR from a moderate thermophile (CAR). It was the first work reported on immobilizing bacterial CAR onto a polymeric support, Seplite LX120, via simple adsorption. Immobilization time and protein load were optimized for CAR immobilization. The immobilized CAR showed optimal activity at 60 °C and pH 9. It was stable over a wide range of temperatures (10 to 100 °C) and pHs (4-11), retaining more than 50% of its activity. The immobilized CAR also showed stability in polar solvents. The adsorption of CAR onto the support was confirmed by Scanning Electron Microscopy (SEM), Fourier-Transform Infrared (FTIR) spectroscopy, and Brunauer-Emmett-Teller (BET) analysis. The immobilized CAR could be stored for up to 6 weeks at 4 °C and 3 weeks at 25 °C. Immobilized CAR showed great operational stability, as 59.68% of its activity was preserved after 10 assay cycles. The immobilized CAR could also convert approximately 2.6 mM of benzoic acid to benzaldehyde at 60 °C. The successfully immobilized CAR on Seplite LX120 exhibited improved properties that benefit green industrial processes.

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