Peel Powder Reduces Intestinal Barrier Defects and Inflammation in a Colitic Murine Experimental Model

Overview

Journal Foods

Specialty Biotechnology

Date 2021 Jan 28

PMID 33503995

Citations 9

Authors

Nguyen Thi Thanh Tinh

Gertrude Cynthia Sitolo

Yoshinari Yamamoto

Takuya Suzuki

Affiliations

Soon will be listed here.

Abstract

This study examines the ameliorative effects of lemon () peel (LP) powder on intestinal inflammation and barrier defects in dextran sulfate sodium (DSS)-induced colitic mice. The whole LP powder was fractionated into methanol (MetOH) extract and its extraction residue (MetOH residue), which were rich in polyphenolic compounds and dietary fibers, respectively. Mice were fed diets containing whole LP powder, MetOH extract, and MetOH residue for 16 d. DSS administration for 9 d induced bodyweight loss, reduced colon length, reduced the colonic expression of tight junction proteins including zonula occludens-1 and -2, and claudin-3 and -7, and upregulated colonic mRNA expression of interleukin 6, chemokine (C-X-C motif) ligand 2, and C-C motif chemokine ligand 2. Feeding LP powder restored these abnormalities, and the MetOH residue, but not MetOH extract, also showed similar restorations. Feeding LP powder and MetOH residue increased fecal concentrations of acetate and n-butyrate. Taken together, LP powder reduced intestinal damage through the protection of tight junction barriers and suppressed an inflammatory reaction in colitic mice. These results suggest that acetate and n-butyrate produced from the microbial metabolism of dietary fibers in LP powder contributed to reducing colitis.

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References

Yan H, Ajuwon K . Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway. PLoS One. 2017; 12(6):e0179586. PMC: 5487041. DOI: 10.1371/journal.pone.0179586. View

Reeves P, Nielsen F, Fahey Jr G . AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993; 123(11):1939-51. DOI: 10.1093/jn/123.11.1939. View

Zheng Y, Zhang R, Shi W, Li L, Liu H, Chen Z . Metabolism and pharmacological activities of the natural health-benefiting compound diosmin. Food Funct. 2020; 11(10):8472-8492. DOI: 10.1039/d0fo01598a. View

Hanauer S . Inflammatory bowel disease. N Engl J Med. 1996; 334(13):841-8. DOI: 10.1056/NEJM199603283341307. View

Abe H, Ishioka M, Fujita Y, Umeno A, Yasunaga M, Sato A . Yuzu (Citrus junos Tanaka) Peel Attenuates Dextran Sulfate Sodium-induced Murine Experimental Colitis. J Oleo Sci. 2018; 67(3):335-344. DOI: 10.5650/jos.ess17184. View

Funaguchi N, Ohno Y, Bai La B, Asai T, Yuhgetsu H, Sawada M . Narirutin inhibits airway inflammation in an allergic mouse model. Clin Exp Pharmacol Physiol. 2007; 34(8):766-70. DOI: 10.1111/j.1440-1681.2007.04636.x. View

Lynch J, Barbano D . Kjeldahl nitrogen analysis as a reference method for protein determination in dairy products. J AOAC Int. 1999; 82(6):1389-98. View

Kelly C, Zheng L, Campbell E, Saeedi B, Scholz C, Bayless A . Crosstalk between Microbiota-Derived Short-Chain Fatty Acids and Intestinal Epithelial HIF Augments Tissue Barrier Function. Cell Host Microbe. 2015; 17(5):662-71. PMC: 4433427. DOI: 10.1016/j.chom.2015.03.005. View

Han J, Lin K, Sequeira C, Borchers C . An isotope-labeled chemical derivatization method for the quantitation of short-chain fatty acids in human feces by liquid chromatography-tandem mass spectrometry. Anal Chim Acta. 2014; 854:86-94. DOI: 10.1016/j.aca.2014.11.015. View

10.

Gonzalez-Molina E, Dominguez-Perles R, Moreno D, Garcia-Viguera C . Natural bioactive compounds of Citrus limon for food and health. J Pharm Biomed Anal. 2009; 51(2):327-45. DOI: 10.1016/j.jpba.2009.07.027. View

11.

Suzuki T . Regulation of the intestinal barrier by nutrients: The role of tight junctions. Anim Sci J. 2020; 91(1):e13357. PMC: 7187240. DOI: 10.1111/asj.13357. View

12.

Cummings J, Macfarlane G, Macfarlane S . Intestinal bacteria and ulcerative colitis. Curr Issues Intest Microbiol. 2003; 4(1):9-20. View

13.

Thiex N . Evaluation of analytical methods for the determination of moisture, crude protein, crude fat, and crude fiber in distillers dried grains with solubles. J AOAC Int. 2009; 92(1):61-73. View

14.

Guo G, Shi W, Shi F, Gong W, Li F, Zhou G . Anti-inflammatory effects of eriocitrin against the dextran sulfate sodium-induced experimental colitis in murine model. J Biochem Mol Toxicol. 2019; 33(11):e22400. DOI: 10.1002/jbt.22400. View

15.

Farooq S, Stillie R, Svensson M, Svanborg C, Strieter R, Stadnyk A . Therapeutic effect of blocking CXCR2 on neutrophil recruitment and dextran sodium sulfate-induced colitis. J Pharmacol Exp Ther. 2009; 329(1):123-9. DOI: 10.1124/jpet.108.145862. View

16.

Huda-Faujan N, Abdulamir A, Fatimah A, Anas O, Shuhaimi M, Yazid A . The impact of the level of the intestinal short chain Fatty acids in inflammatory bowel disease patients versus healthy subjects. Open Biochem J. 2010; 4:53-8. PMC: 2887640. DOI: 10.2174/1874091X01004010053. View

17.

Loubinoux J, Bronowicki J, Pereira I, Mougenel J, Faou A . Sulfate-reducing bacteria in human feces and their association with inflammatory bowel diseases. FEMS Microbiol Ecol. 2009; 40(2):107-12. DOI: 10.1111/j.1574-6941.2002.tb00942.x. View

18.

Suzuki T, Yoshida S, Hara H . Physiological concentrations of short-chain fatty acids immediately suppress colonic epithelial permeability. Br J Nutr. 2008; 100(2):297-305. DOI: 10.1017/S0007114508888733. View

19.

Dordevic D, Jancikova S, Vitezova M, Kushkevych I . Hydrogen sulfide toxicity in the gut environment: Meta-analysis of sulfate-reducing and lactic acid bacteria in inflammatory processes. J Adv Res. 2020; 27:55-69. PMC: 7728594. DOI: 10.1016/j.jare.2020.03.003. View

20.

Suzuki T, Yoshinaga N, Tanabe S . Interleukin-6 (IL-6) regulates claudin-2 expression and tight junction permeability in intestinal epithelium. J Biol Chem. 2011; 286(36):31263-71. PMC: 3173073. DOI: 10.1074/jbc.M111.238147. View