CircSKA3 Downregulates MiR-1 Through Methylation in Glioblastoma to Promote Cancer Cell Proliferation

Overview

Journal Cancer Manag Res

Publisher Dove Medical Press

Specialty Oncology

Date 2021 Jan 27

PMID 33500664

Citations 8

Authors

Meng Zhou

Huan Li

Keen Chen

Weilong Ding

Chengyou Yang

Xiangyu Wang

Affiliations

Soon will be listed here.

Abstract

Background: Circular RNA circSKA3 plays an oncogenic role in breast cancer, while its role in glioblastoma (GBM) is unknown. This study aimed to explore the role of circSKA3 in GBM.

Methods: Differential expression of circSKA3 and miR-1 in GBM and adjacent non-cancer tissue samples were analyzed by RT-qPCR. GBM cells were transfected with circSKA3 expression vector or miR-1 mimic, followed by RT-qPCR to explore the potential crosstalk between them. Methylation-specific PCR (MSP) was carried out to assess the role of circSKA3 in regulating the methylation of miR-1 gene. The role of circSKA3 and miR-1 in regulating GBM cell proliferation was analyzed by CCK-8 assay.

Results: We found that circSKA3 was upregulated in GBM and inversely correlated with miR-1 across GBM tissues. High expression levels of circSKA3 and low expression levels of miR-1 were significantly correlated with the poor survival of GBM patients. In GBM cells, overexpression of circSKA3 increased the methylation of miR-1 gene and decreased the expression of miR-1. CCK-8 assay showed that overexpression of circSKA3 reduced the inhibitory effects of miR-1 on cell proliferation.

Conclusion: Therefore, circSKA3 may downregulate miR-1 through methylation in GBM to promote cancer cell proliferation.

Citing Articles

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Specific intracellular retention of circSKA3 promotes colorectal cancer metastasis by attenuating ubiquitination and degradation of SLUG.

Deng J, Liao S, Chen C, Han F, Lei S, Lai X Cell Death Dis. 2023; 14(11):750.

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The important role of miR-1-3p in cancers.

Dai S, Li F, Xu S, Hu J, Gao L J Transl Med. 2023; 21(1):769.

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The Glioblastoma CircularRNAome.

Tirpe A, Streianu C, Tirpe S, Kocijancic A, Pirlog R, Pirlog B Int J Mol Sci. 2023; 24(19).

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Insights into the role of long non-coding RNAs in DNA methylation mediated transcriptional regulation.

Yang Z, Xu F, Teschendorff A, Zhao Y, Yao L, Li J Front Mol Biosci. 2022; 9:1067406.

PMID: 36533073 PMC: 9755597. DOI: 10.3389/fmolb.2022.1067406.

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