Bioengineering of Adenylate Cyclase Toxin for Vaccine Development and Other Biotechnological Purposes

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2021 Jan 27

PMID 33499260

Citations 3

Authors

Daniel Ladant

Affiliations

Soon will be listed here.

Abstract

The adenylate cyclase toxin, CyaA, is one of the key virulent factors produced by , the causative agent of whooping cough. This toxin primarily targets innate immunity to facilitate bacterial colonization of the respiratory tract. CyaA exhibits several remarkable characteristics that have been exploited for various applications in vaccinology and other biotechnological purposes. CyaA has been engineered as a potent vaccine vehicle to deliver antigens into antigen-presenting cells, while the adenylate cyclase catalytic domain has been used to design a robust genetic assay for monitoring protein-protein interactions in bacteria. These two biotechnological applications are briefly summarized in this chapter.

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