Pertussis Toxin and Adenylate Cyclase Toxin: Key Virulence Factors of Bordetella Pertussis and Cell Biology Tools

Overview

Journal Future Microbiol

Specialties Infectious Diseases
Microbiology

Date 2010 Mar 10

PMID 20210554

Citations 99

Authors

Nicholas H Carbonetti

Affiliations

Soon will be listed here.

Abstract

Pertussis toxin and adenylate cyclase toxin are two important virulence factors of Bordetella pertussis, the bacterial cause of the respiratory disease pertussis or whooping cough. In addition to studies on the structure, function and role in pathogenesis of these two toxins, they are both used as cell biology tools for a variety of applications owing to their ability to enter mammalian cells, perform enzymatic activities and modify cell signaling events. In this article, recent data from the research literature that enhance our understanding of the nature of these two toxins, their role in the pathogenesis of B. pertussis infection and disease, particularly in modulating host immune responses, and their use as tools for other areas of research will be outlined.

Citing Articles

Hydroxyurea for Malignant Pertussis in Critically Ill Children.

Blanc M, Marais C, Debs A, Cousin V, Tissieres P Crit Care Explor. 2025; 7(2):e1218.

PMID: 39937591 PMC: 11826043. DOI: 10.1097/CCE.0000000000001218.

The diverse landscape of AB5-type toxins.

Brown P, Ojiakor A, Chemello A, Fowler C Eng Microbiol. 2024; 3(4):100104.

PMID: 39628907 PMC: 11610972. DOI: 10.1016/j.engmic.2023.100104.

Modeling Lymphoma Angiogenesis, Lymphangiogenesis, and Vessel Co-Option, and the Effects of Inhibition of Lymphoma-Vessel Interactions with an αCD20-EndoP125A Antibody Fusion Protein.

Elledge C, Zhang Y, Shin S, Cho H, Ramakrishnan S, Sankar A Cells. 2024; 13(22).

PMID: 39594584 PMC: 11593053. DOI: 10.3390/cells13221835.

A deep learning method to predict bacterial ADP-ribosyltransferase toxins.

Zheng D, Zhou S, Chen L, Pang G, Yang J Bioinformatics. 2024; 40(7).

PMID: 38885365 PMC: 11219481. DOI: 10.1093/bioinformatics/btae378.

Safety assessments of recombinant DTaP vaccines developed in South Korea.

Choi G, Kang K, Kim S, Ji J, Cho G, Kang H Clin Exp Vaccine Res. 2024; 13(2):155-165.

PMID: 38752005 PMC: 11091433. DOI: 10.7774/cevr.2024.13.2.155.

References

Mascarell L, Bauche C, Fayolle C, Diop O, Dupuy M, Nougarede N . Delivery of the HIV-1 Tat protein to dendritic cells by the CyaA vector induces specific Th1 responses and high affinity neutralizing antibodies in non human primates. Vaccine. 2006; 24(17):3490-9. DOI: 10.1016/j.vaccine.2006.02.009. View

Spensieri F, Fedele G, Fazio C, Nasso M, Stefanelli P, Mastrantonio P . Bordetella pertussis inhibition of interleukin-12 (IL-12) p70 in human monocyte-derived dendritic cells blocks IL-12 p35 through adenylate cyclase toxin-dependent cyclic AMP induction. Infect Immun. 2006; 74(5):2831-8. PMC: 1459734. DOI: 10.1128/IAI.74.5.2831-2838.2006. View

Trollfors B, TARANGER J, Lagergard T, Lind L, Sundh V, Zackrisson G . A placebo-controlled trial of a pertussis-toxoid vaccine. N Engl J Med. 1995; 333(16):1045-50. DOI: 10.1056/NEJM199510193331604. View

Khelef N, Guiso N . Induction of macrophage apoptosis by Bordetella pertussis adenylate cyclase-hemolysin. FEMS Microbiol Lett. 1995; 134(1):27-32. DOI: 10.1111/j.1574-6968.1995.tb07909.x. View

Hazes B, Boodhoo A, Cockle S, Read R . Crystal structure of the pertussis toxin-ATP complex: a molecular sensor. J Mol Biol. 1996; 258(4):661-71. DOI: 10.1006/jmbi.1996.0277. View

Lilie H, Haehnel W, Rudolph R, Baumann U . Folding of a synthetic parallel beta-roll protein. FEBS Lett. 2000; 470(2):173-7. DOI: 10.1016/s0014-5793(00)01308-9. View

Weingart C, Hewlett E, Gray M, Weiss A . Neutralizing antibodies to adenylate cyclase toxin promote phagocytosis of Bordetella pertussis by human neutrophils. Infect Immun. 2000; 68(12):7152-5. PMC: 97830. DOI: 10.1128/IAI.68.12.7152-7155.2000. View

Chenal A, Guijarro J, Raynal B, Delepierre M, Ladant D . RTX calcium binding motifs are intrinsically disordered in the absence of calcium: implication for protein secretion. J Biol Chem. 2008; 284(3):1781-9. DOI: 10.1074/jbc.M807312200. View

Andreasen C, Carbonetti N . Role of neutrophils in response to Bordetella pertussis infection in mice. Infect Immun. 2008; 77(3):1182-8. PMC: 2643619. DOI: 10.1128/IAI.01150-08. View

10.

Robbins J, Schneerson R, Keith J, Miller M, Kubler-Kielb J, Trollfors B . Pertussis vaccine: a critique. Pediatr Infect Dis J. 2009; 28(3):237-41. PMC: 3128455. DOI: 10.1097/INF.0b013e31818a8958. View

11.

Rossi Paccani S, Benagiano M, Capitani N, Zornetta I, Ladant D, Montecucco C . The adenylate cyclase toxins of Bacillus anthracis and Bordetella pertussis promote Th2 cell development by shaping T cell antigen receptor signaling. PLoS Pathog. 2009; 5(3):e1000325. PMC: 2643477. DOI: 10.1371/journal.ppat.1000325. View

12.

Schneider O, Weiss A, Miller W . Pertussis toxin signals through the TCR to initiate cross-desensitization of the chemokine receptor CXCR4. J Immunol. 2009; 182(9):5730-9. PMC: 2766007. DOI: 10.4049/jimmunol.0803114. View

13.

Pierce C, Klein N, Peters M . Is leukocytosis a predictor of mortality in severe pertussis infection?. Intensive Care Med. 2000; 26(10):1512-4. DOI: 10.1007/s001340000587. View

14.

Guermonprez P, Khelef N, Blouin E, Rieu P, Ricciardi-Castagnoli P, Guiso N . The adenylate cyclase toxin of Bordetella pertussis binds to target cells via the alpha(M)beta(2) integrin (CD11b/CD18). J Exp Med. 2001; 193(9):1035-44. PMC: 2193436. DOI: 10.1084/jem.193.9.1035. View

15.

Mills K . Immunity to Bordetella pertussis. Microbes Infect. 2001; 3(8):655-77. DOI: 10.1016/s1286-4579(01)01421-6. View

16.

Dautin N, Karimova G, Ladant D . Bordetella pertussis adenylate cyclase toxin: a versatile screening tool. Toxicon. 2002; 40(10):1383-7. DOI: 10.1016/s0041-0101(02)00158-7. View

17.

El-Azami-El-Idrissi M, Bauche C, Loucka J, Osicka R, Sebo P, Ladant D . Interaction of Bordetella pertussis adenylate cyclase with CD11b/CD18: Role of toxin acylation and identification of the main integrin interaction domain. J Biol Chem. 2003; 278(40):38514-21. DOI: 10.1074/jbc.M304387200. View

18.

Carbonetti N, Artamonova G, Mays R, Worthington Z . Pertussis toxin plays an early role in respiratory tract colonization by Bordetella pertussis. Infect Immun. 2003; 71(11):6358-66. PMC: 219603. DOI: 10.1128/IAI.71.11.6358-6366.2003. View

19.

Mikelova L, Halperin S, Scheifele D, Smith B, Ford-Jones E, Vaudry W . Predictors of death in infants hospitalized with pertussis: a case-control study of 16 pertussis deaths in Canada. J Pediatr. 2003; 143(5):576-81. DOI: 10.1067/S0022-3476(03)00365-2. View

20.

Rizzi C, Alfano M, Bugatti A, Camozzi M, Poli G, Rusnati M . Inhibition of intra- and extra-cellular Tat function and HIV expression by pertussis toxin B-oligomer. Eur J Immunol. 2004; 34(2):530-6. DOI: 10.1002/eji.200324142. View