Human Pluripotent Stem Cell-derived DDX4 and KRT-8 Positive Cells Participate in Ovarian Follicle-like Structure Formation

Overview

Journal iScience

Publisher Cell Press

Date 2021 Jan 25

PMID 33490911

Citations 6

Authors

Danny C W Yu

Fang-Chun Wu

Chia-Eng Wu

Lu-Ping Chow

Hong-Nerng Ho

Hsin-Fu Chen

Affiliations

Soon will be listed here.

Abstract

Understanding the mechanisms of human pluripotent stem cells (hPSCs) specification, development and differentiation to gametes are useful for elucidating the causes of infertility and potential treatment. This study aims to examine whether hPSCs can be induced to DDX4 extracellularly expressing primordial germ cell-like cells (DDX4 PGCLCs) and further into ovarian follicle stage in a combined and model. The transcriptional signatures show that these DDX4 PGCLCs are characteristic of PGCs and express ovarian folliculogenesis markers. We also verify that keratin (KRT)-8 is highly expressed in the DDX4 PGCLCs and plays a crucial role in germ cell migration. By co-culturing DDX4 PGCLCs with human granulosa cells (GCs), these cells are further induced into ovarian follicle-like structures in a xenograft mice model. This approach can in the future design practical strategies for treating germ cell-associated issues of infertility.

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