Microbial Risk Assessment of in Polluted Environments, Case of Urban Rainfall Water

Objectives: To assess the spatio-temporal dynamics of pathogenic in UIBs and to stablish phylogenetic relationships between clinical and UIB strains. To assess pathogenicity associated with environmental using an animal model, and to identify genetic elements that may be associated to its virulence.

Methods: A well-characterized UIB in terms of chemical pollutants from Lyon area was used in this study during a whole year. Cultural and Next-Generation-Sequencing methods were used for detection and typing. Clinical and environmental isolates phylogenetic relationships and virulences were compared with Multilocus-Sequence-Analysis study together with a murine model.

Results: In autumn, and were the pathogenic most prevalent species in the UIB The complex was also detected together with some other non-pathogenic species. The presence of pathogenic was positively correlated to metallic trace elements. Up to 1.0 × 10 CFU/g sediment of and 6 OTUs splited in two different phylogroups were retrieved and were close to clinical strains. The EML446 tested UIB isolate showed significant infectivity in mice with pulmonary damages similar to clinical clone (GUH-2).

Conclusion: marker-based metabarcoding approach allowed detecting as the most abundant pathogenic species in a UIB. Metal trace elements-polluted environments can be reservoirs of pathogenic which may have a similar virulence to clinical strains.