Antibacterial Properties of on Intracanal Medicaments Against Biofilm at Different Stages of Development

Background: Use of herbal compounds as an intracanal medicament in the field of endodontics has become noteworthy, one of which is the compound whose antibacterial effect has already been proven in the planktonic form of . The purpose of this study is to evaluate the antibacterial effect of on biofilms at the 4 and 6 week of development.

Materials And Methods: 130 single root canal teeth without anomalies and caries were used. They were divided into two groups of 65 teeth for four and six weeks of biofilm production. Five samples of each group were examined for confirmation of biofilm formation under an electron microscope. Study groups were investigated with an antimicrobial agent as an intracanal medicament including 20 samples treated with , calcium hydroxide, and phosphate-buffered saline, and biofilm and survival of pathogens were investigated. Dentin chip suspensions were used for colony-forming unit (CFU) counting to estimate remaining counts.

Results: The CFU mean in the 4 week subgroup in , phosphate-buffered saline, and calcium hydroxide was 0, 69166.66 ± 31688.58, and 25000 ± 30822.07, and in the 6 week, it was 136.36 ± 323.33, 95000 ± 12247.44, and 27501.66 ± 36570.34, respectively, which showed a significant difference between the used materials ( < 0.05).

Conclusion: , in contrast to calcium hydroxide, eliminated 4 and 6 week biofilms and showed remarkable antibacterial properties against biofilm. These results support potency of to use as a natural antimicrobial material in the intracanal medicament.