Molecular Characterization of Cryptosporidium Isolates from Humans in Ontario, Canada

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2021 Jan 23

PMID 33482898

Citations 16

Authors

Rebecca A Guy

Christine A Yanta

Pia K Muchaal

Marisa A Rankin

Karine Thivierge

Rachel Lau

Andrea K Boggild

Affiliations

Soon will be listed here.

Abstract

Background: Cryptosporidiosis is a gastrointestinal disease with global distribution. It has been a reportable disease in Canada since 2000; however, routine molecular surveillance is not conducted. Therefore, sources of contamination are unknown. The aim of this project was to identify species and subtypes of Cryptosporidium in clinical cases from Ontario, the largest province in Canada, representing one third of the Canadian population, in order to understand transmission patterns.

Methods: A total of 169 frozen, banked, unpreserved stool specimens that were microscopy positive for Cryptosporidium over the period 2008-2017 were characterized using molecular tools. A subset of the 169 specimens were replicate samples from individual cases. DNA was extracted directly from the stool and nested PCR followed by Sanger sequencing was conducted targeting the small subunit ribosomal RNA (SSU) and glycoprotein 60 (gp60) genes.

Results: Molecular typing data and limited demographic data were obtained for 129 cases of cryptosporidiosis. Of these cases, 91 (70.5 %) were due to Cryptosporidium parvum and 24 (18.6%) were due to Cryptosporidium hominis. Mixed infections of C. parvum and C. hominis occurred in four (3.1%) cases. Five other species observed were Cryptosporidium ubiquitum (n = 5), Cryptosporidium felis (n = 2), Cryptosporidium meleagridis (n = 1), Cryptosporidium cuniculus (n = 1) and Cryptosporidium muris (n = 1). Subtyping the gp60 gene revealed 5 allelic families and 17 subtypes of C. hominis and 3 allelic families and 17 subtypes of C. parvum. The most frequent subtype of C. hominis was IbA10G2 (22.3%) and of C. parvum was IIaA15G2R1 (62.4%).

Conclusions: The majority of isolates in this study were C. parvum, supporting the notion that zoonotic transmission is the main route of cryptosporidiosis transmission in Ontario. Nonetheless, the observation of C. hominis in about a quarter of cases suggests that anthroponotic transmission is also an important contributor to cryptosporidiosis pathogenesis in Ontario.

Citing Articles

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Cryptosporidium parvum: an emerging occupational zoonosis in Finland.

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References

Ng-Hublin J, Combs B, Reid S, Ryan U . Comparison of three cryptosporidiosis outbreaks in Western Australia: 2003, 2007 and 2011. Epidemiol Infect. 2018; 146(11):1413-1424. PMC: 9133686. DOI: 10.1017/S0950268818001607. View

Peng M, Wilson M, Holland R, Meshnick S, Lal A, Xiao L . Genetic diversity of Cryptosporidium spp. in cattle in Michigan: implications for understanding the transmission dynamics. Parasitol Res. 2003; 90(3):175-80. DOI: 10.1007/s00436-003-0834-5. View

. Laboratory-based surveillance for Cryptosporidium in France, 2006-2009. Euro Surveill. 2010; 15(33):19642. View

Feltus D, Giddings C, Schneck B, Monson T, Warshauer D, McEvoy J . Evidence supporting zoonotic transmission of Cryptosporidium spp. in Wisconsin. J Clin Microbiol. 2006; 44(12):4303-8. PMC: 1698413. DOI: 10.1128/JCM.01067-06. View

Khalil I, Troeger C, Rao P, Blacker B, Brown A, Brewer T . Morbidity, mortality, and long-term consequences associated with diarrhoea from Cryptosporidium infection in children younger than 5 years: a meta-analyses study. Lancet Glob Health. 2018; 6(7):e758-e768. PMC: 6005120. DOI: 10.1016/S2214-109X(18)30283-3. View

Nic Lochlainn L, Sane J, Schimmer B, Mooij S, Roelfsema J, van Pelt W . Risk Factors for Sporadic Cryptosporidiosis in the Netherlands: Analysis of a 3-Year Population Based Case-Control Study Coupled With Genotyping, 2013-2016. J Infect Dis. 2018; 219(7):1121-1129. PMC: 6420163. DOI: 10.1093/infdis/jiy634. View

Caccio S, Chalmers R . Human cryptosporidiosis in Europe. Clin Microbiol Infect. 2016; 22(6):471-80. DOI: 10.1016/j.cmi.2016.04.021. View

Insulander M, Silverlas C, Lebbad M, Karlsson L, Mattsson J, Svenungsson B . Molecular epidemiology and clinical manifestations of human cryptosporidiosis in Sweden. Epidemiol Infect. 2012; 141(5):1009-20. PMC: 9151846. DOI: 10.1017/S0950268812001665. View

Xiao L, Hlavsa M, Yoder J, Ewers C, Dearen T, Yang W . Subtype analysis of Cryptosporidium specimens from sporadic cases in Colorado, Idaho, New Mexico, and Iowa in 2007: widespread occurrence of one Cryptosporidium hominis subtype and case history of an infection with the Cryptosporidium horse genotype. J Clin Microbiol. 2009; 47(9):3017-20. PMC: 2738111. DOI: 10.1128/JCM.00226-09. View

10.

Yanta C, Bessonov K, Robinson G, Troell K, Guy R . CryptoGenotyper: A new bioinformatics tool for rapid identification. Food Waterborne Parasitol. 2021; 23:e00115. PMC: 7966988. DOI: 10.1016/j.fawpar.2021.e00115. View

11.

Appelbee A, Thompson R, Measures L, Olson M . Giardia and Cryptosporidium in harp and hooded seals from the Gulf of St. Lawrence, Canada. Vet Parasitol. 2010; 173(1-2):19-23. DOI: 10.1016/j.vetpar.2010.06.001. View

12.

Chalmers R, Smith R, Hadfield S, Elwin K, Giles M . Zoonotic linkage and variation in Cryptosporidium parvum from patients in the United Kingdom. Parasitol Res. 2011; 108(5):1321-5. DOI: 10.1007/s00436-010-2199-x. View

13.

Thivierge K, Iqbal A, Dixon B, Dion R, Levesque B, Cantin P . Cryptosporidium hominis Is a Newly Recognized Pathogen in the Arctic Region of Nunavik, Canada: Molecular Characterization of an Outbreak. PLoS Negl Trop Dis. 2016; 10(4):e0004534. PMC: 4825996. DOI: 10.1371/journal.pntd.0004534. View

14.

Moore C, Elwin K, Phot N, Seng C, Mao S, Suy K . Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children. PLoS Negl Trop Dis. 2016; 10(7):e0004822. PMC: 4936737. DOI: 10.1371/journal.pntd.0004822. View

15.

Hutter J, Dion R, Irace-Cima A, Fiset M, Guy R, Dixon B . Cryptosporidium spp.: Human incidence, molecular characterization and associated exposures in Québec, Canada (2016-2017). PLoS One. 2020; 15(2):e0228986. PMC: 7018055. DOI: 10.1371/journal.pone.0228986. View

16.

Sannella A, Suputtamongkol Y, Wongsawat E, Caccio S . A retrospective molecular study of Cryptosporidium species and genotypes in HIV-infected patients from Thailand. Parasit Vectors. 2019; 12(1):91. PMC: 6417249. DOI: 10.1186/s13071-019-3348-4. View

17.

Xiao L, Alderisio K, Limor J, Royer M, Lal A . Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Appl Environ Microbiol. 2000; 66(12):5492-8. PMC: 92489. DOI: 10.1128/AEM.66.12.5492-5498.2000. View

18.

Xiao L . Molecular epidemiology of cryptosporidiosis: an update. Exp Parasitol. 2009; 124(1):80-9. DOI: 10.1016/j.exppara.2009.03.018. View

19.

Liao C, Wang T, Koehler A, Fan Y, Hu M, Gasser R . Molecular investigation of Cryptosporidium in farmed chickens in Hubei Province, China, identifies 'zoonotic' subtypes of C. meleagridis. Parasit Vectors. 2018; 11(1):484. PMC: 6114272. DOI: 10.1186/s13071-018-3056-5. View

20.

Ng J, Mackenzie B, Ryan U . Longitudinal multi-locus molecular characterisation of sporadic Australian human clinical cases of cryptosporidiosis from 2005 to 2008. Exp Parasitol. 2010; 125(4):348-56. DOI: 10.1016/j.exppara.2010.02.017. View