Delivery of the 5-HT Receptor Agonist, DOI, Enhances Activity of the Sphincter Muscle During the Micturition Reflex in Rats After Spinal Cord Injury

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2021 Jan 22

PMID 33477834

Citations 2

Authors

Jaclyn H DeFinis

Jeremy Weinberger

Shaoping Hou

Affiliations

Soon will be listed here.

Abstract

Traumatic spinal cord injury (SCI) interrupts spinobulbospinal micturition reflex pathways and results in urinary dysfunction. Over time, an involuntary bladder reflex is established due to the reorganization of spinal circuitry. Previous studies show that manipulation of serotonin 2A (5-HT) receptors affects recovered bladder function, but it remains unclear if this receptor regulates the activity of the external urethral sphincter (EUS) following SCI. To elucidate how central and peripheral serotonergic machinery acts on the lower urinary tract (LUT) system, we employed bladder cystometry and EUS electromyography recordings combined with intravenous or intrathecal pharmacological interventions of 5-HT receptors in female SCI rats. Three to four weeks after a T10 spinal transection, systemic and central blockage of 5-HT receptors with MDL only slightly influenced the micturition reflex. However, delivery of the 5-HT receptor agonist, DOI, increased EUS tonic activity and elicited bursting during voiding. Additionally, subcutaneous administration of DOI verified the enhancement of continence and voiding capability during spontaneous micturition in metabolic cage assays. Although spinal 5HT receptors may not be actively involved in the recovered micturition reflex, stimulating this receptor subtype enhances EUS function and the synergistic activity between the detrusor and sphincter to improve the micturition reflex in rats with SCI.

Citing Articles

Upregulated 5-HT Receptors Regulate Lower Urinary Tract Function in Rats after Complete Spinal Cord Injury.

Lin C, Li K, Thalluri R, Lee Y J Neurotrauma. 2023; 40(9-10):845-861.

PMID: 36762948 PMC: 10162122. DOI: 10.1089/neu.2022.0329.

Locomotor Exercise Enhances Supraspinal Control of Lower-Urinary-Tract Activity to Improve Micturition Function after Contusive Spinal-Cord Injury.

Deng L, Sui T, Wang D, Hou S, Cao X, Peng K Cells. 2022; 11(9).

PMID: 35563703 PMC: 9104392. DOI: 10.3390/cells11091398.

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