Extraction of DNA from Micro-tissue for Bat Species Identification

Overview

Journal Mitochondrial DNA B Resour

Date 2021 Jan 21

PMID 33474313

Authors

Thais Fernandes Mendonca Mota

Thomaz Mansini Carrenho Fabrin

Luciano Seraphim Gasques

Henrique Ortencio Filho

Alberto Jose Prioli

Sonia Maria Alves Pinto Prioli

Affiliations

Soon will be listed here.

Abstract

Bat populations are declining worldwide. Accurate identification is essential to promote species' conservation. However, minimal morphological differences and a high rate of cryptic species make identification difficult, unless voucher specimens are kept, a controversial issue today. The objective of this work was to standardize a method of extracting non-lethal DNA using bats' uropatagium micro-tissue, aiming the molecular identification of species that occur in the region of Maringá PR. The method standardized was efficient, and does not cause serious damage to bats. For future field studies, collection of micro-tissue and morphometry of the specimens will be sufficient for accurate identification.

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