The Role and Mechanism of the Annexin A1 Peptide Ac2-26 in Rats with Cardiopulmonary Bypass Lung Injury

Overview

Journal Basic Clin Pharmacol Toxicol

Specialties Pharmacology
Toxicology

Date 2021 Jan 17

PMID 33455036

Citations 3

Authors

Jiyang Xu

Chengkun Yu

Junli Luo

Yuhan Guo

Chi Cheng

Hong Zhang

Affiliations

Soon will be listed here.

Abstract

The main causes of lung injury after cardiopulmonary bypass (CPB) are systemic inflammatory response syndrome (SIRS) and pulmonary ischaemia-reperfusion injury (IR-I). SIRS and IR-I are often initiated by a systemic inflammatory response. The present study investigated whether the annexin A1 (ANX-A1) peptidomimetic Ac2-26 by binding to formyl peptide receptors (FPRs) inhibit inflammatory cytokines and reduce lung injury after CPB. Male rats were randomized to the following five groups (n = 6, each): sham, exposed to pulmonary ischaemic-reperfusion (IR-I), IR-I plus Ac2-26, IR-I plus the FPR antagonist, BoC2 (N-tert-butyloxycarbonyl-Phe-Leu-Phe-Leu-Phe) and IR-I plus Ac2-26 and BoC2. Treatment with Ac2-26 improved the oxygenation index, an effect blocked by BoC2. Histopathological analysis of the lung tissue revealed that the degree of lung injury was significantly less (P < 0.05) in the Ac2-26-treated rats compared to the other experimental groups exposed to IR-I. Ac2-26 treatment reduced the levels of the inflammatory cytokines TNF-α, IL-1β, ICAM-1 and NF-κB-p65 (P < 0.05) compared to the vehicle-treated group exposed to IR-I. In conclusion, the annexin A1 (ANX-A1) peptidomimetic Ac2-26 by binding to formyl peptide receptors inhibit inflammatory cytokines and reduce ischaemic-reperfusion lung injury after cardiopulmonary bypass.

Citing Articles

ANNEXIN A1: Roles in Placenta, Cell Survival, and Nucleus.

de Sousa S, Santos M, Teixeira S, Ferro E, Oliani S Cells. 2022; 11(13).

PMID: 35805141 PMC: 9266233. DOI: 10.3390/cells11132057.

Narrative review of the systemic inflammatory reaction to cardiac surgery and cardiopulmonary bypass.

Squiccimarro E, Stasi A, Lorusso R, Paparella D Artif Organs. 2022; 46(4):568-577.

PMID: 35061922 PMC: 9303696. DOI: 10.1111/aor.14171.

The role and mechanism of the annexin A1 peptide Ac2-26 in rats with cardiopulmonary bypass lung injury.

Xu J, Yu C, Luo J, Guo Y, Cheng C, Zhang H Basic Clin Pharmacol Toxicol. 2021; 128(6):719-730.

PMID: 33455036 PMC: 8247988. DOI: 10.1111/bcpt.13561.

References

Yu Y, Gao M, Li H, Zhang F, Gu C . Pulmonary artery perfusion with anti-tumor necrosis factor alpha antibody reduces cardiopulmonary bypass-induced inflammatory lung injury in a rabbit model. PLoS One. 2014; 8(12):e83236. PMC: 3873915. DOI: 10.1371/journal.pone.0083236. View

Spurr L, Nadkarni S, Pederzoli-Ribeil M, Goulding N, Perretti M, DAcquisto F . Comparative analysis of Annexin A1-formyl peptide receptor 2/ALX expression in human leukocyte subsets. Int Immunopharmacol. 2010; 11(1):55-66. DOI: 10.1016/j.intimp.2010.10.006. View

Guay J, Kopp S . Epidural analgesia for adults undergoing cardiac surgery with or without cardiopulmonary bypass. Cochrane Database Syst Rev. 2019; 3:CD006715. PMC: 6396869. DOI: 10.1002/14651858.CD006715.pub3. View

Cao Q, Qu M, Yang W, Wang D, Zhang M, Di S . Ischemia postconditioning preventing lung ischemia-reperfusion injury. Gene. 2014; 554(1):120-4. DOI: 10.1016/j.gene.2014.10.009. View

Sugimoto M, Vago J, Teixeira M, Sousa L . Annexin A1 and the Resolution of Inflammation: Modulation of Neutrophil Recruitment, Apoptosis, and Clearance. J Immunol Res. 2016; 2016:8239258. PMC: 4738713. DOI: 10.1155/2016/8239258. View

Perretti M, DAcquisto F . Annexin A1 and glucocorticoids as effectors of the resolution of inflammation. Nat Rev Immunol. 2008; 9(1):62-70. DOI: 10.1038/nri2470. View

Peshavariya H, Taylor C, Goh C, Liu G, Jiang F, Chan E . Annexin peptide Ac2-26 suppresses TNFα-induced inflammatory responses via inhibition of Rac1-dependent NADPH oxidase in human endothelial cells. PLoS One. 2013; 8(4):e60790. PMC: 3634803. DOI: 10.1371/journal.pone.0060790. View

La M, DAmico M, Bandiera S, Di Filippo C, Oliani S, Gavins F . Annexin 1 peptides protect against experimental myocardial ischemia-reperfusion: analysis of their mechanism of action. FASEB J. 2001; 15(12):2247-56. DOI: 10.1096/fj.01-0196com. View

Qin C, Yang Y, May L, Gao X, Stewart A, Tu Y . Cardioprotective potential of annexin-A1 mimetics in myocardial infarction. Pharmacol Ther. 2014; 148:47-65. DOI: 10.1016/j.pharmthera.2014.11.012. View

10.

Tveden-Nyborg P, Bergmann T, Jessen N, Simonsen U, Lykkesfeldt J . BCPT policy for experimental and clinical studies. Basic Clin Pharmacol Toxicol. 2020; 128(1):4-8. DOI: 10.1111/bcpt.13492. View

11.

Michalski M, Pagowska-Klimek I, Thiel S, Swierzko A, Hansen A, Jensenius J . Factors involved in initiation and regulation of complement lectin pathway influence postoperative outcome after pediatric cardiac surgery involving cardiopulmonary bypass. Sci Rep. 2019; 9(1):2930. PMC: 6393526. DOI: 10.1038/s41598-019-39742-w. View

12.

Cheng C, Li S, Wang Y, Chen S, You L, Zhang H . Ischemic postconditioning alleviates lung injury and maintains a better expression of aquaporin-1 during cardiopulmonary bypass. Chin Med J (Engl). 2014; 127(23):4012-8. View

13.

Liu L, An D, Xu J, Shao B, Li X, Shi J . Ac2-26 Induces IKKβ Degradation Through Chaperone-Mediated Autophagy Via HSPB1 in NCM-Treated Microglia. Front Mol Neurosci. 2018; 11:76. PMC: 5890123. DOI: 10.3389/fnmol.2018.00076. View

14.

Lopez B, Maisonet T, Londhe V . Alveolar NF-κB signaling regulates endotoxin-induced lung inflammation. Exp Lung Res. 2014; 41(2):103-14. DOI: 10.3109/01902148.2014.977461. View

15.

Stephens R, Shah A, Whitman G . Lung injury and acute respiratory distress syndrome after cardiac surgery. Ann Thorac Surg. 2013; 95(3):1122-9. DOI: 10.1016/j.athoracsur.2012.10.024. View

16.

Rezzola S, Corsini M, Chiodelli P, Cancarini A, Nawaz I, Coltrini D . Inflammation and N-formyl peptide receptors mediate the angiogenic activity of human vitreous humour in proliferative diabetic retinopathy. Diabetologia. 2017; 60(4):719-728. DOI: 10.1007/s00125-016-4204-0. View

17.

Gao M, Xie B, Gu C, Li H, Zhang F, Yu Y . Targeting the proinflammatory cytokine tumor necrosis factor-α to alleviate cardiopulmonary bypass-induced lung injury (review). Mol Med Rep. 2014; 11(4):2373-8. DOI: 10.3892/mmr.2014.3050. View

18.

Liao W, Wu S, Wu G, Pao H, Tang S, Huang K . Ac2-26, an Annexin A1 Peptide, Attenuates Ischemia-Reperfusion-Induced Acute Lung Injury. Int J Mol Sci. 2017; 18(8). PMC: 5578160. DOI: 10.3390/ijms18081771. View

19.

Guido B, Zanatelli M, Tavares-de-Lima W, Oliani S, Damazo A . Annexin-A1 peptide down-regulates the leukocyte recruitment and up-regulates interleukin-10 release into lung after intestinal ischemia-reperfusion in mice. J Inflamm (Lond). 2013; 10(1):10. PMC: 3608250. DOI: 10.1186/1476-9255-10-10. View

20.

Daufenback Machado I, Spatti M, Hastreiter A, Santin J, Fock R, Gil C . Annexin A1 Is a Physiological Modulator of Neutrophil Maturation and Recirculation Acting on the CXCR4/CXCL12 Pathway. J Cell Physiol. 2016; 231(11):2418-27. DOI: 10.1002/jcp.25346. View