Genetic Diversity and Distribution of Virulence-Associated Genes in and -Like Isolates from Humans and Animals in Poland

Overview

Journal Pathogens

Date 2021 Jan 16

PMID 33450948

Citations 6

Authors

Katarzyna Morka

Ewa Walecka-Zacharska

Justyna Schubert

Bartlomiej Dudek

Anna Wozniak-Biel

Maciej Kuczkowski

Alina Wieliczko

Jaroslaw Bystron

Jacek Bania

Gabriela Bugla-Ploskonska

Affiliations

Soon will be listed here.

Abstract

widespread within domestic and wild-living animals, is a foodborne pathogen causing yersiniosis. The goal of this study was to assess a genetic similarity of and -like strains isolated from different hosts using Multiple Locus Variable-Number Tandem Repeat Analysis (MLVA) and Pulsed-Field Gel Electrophoresis (PFGE) methods, and analyze the prevalence of virulence genes using -Polymerase Chain Reaction (PCR) assays. Among 51 sp. strains 20 virulotypes were determined. The most common virulence genes were , , , , and . sp. strains had genes which may contribute to the bacterial invasion and colonization of the intestines as well as survival in serum. One wild boar 1A strain possessed gene implying the possible pathogenicity of 1A biotype. Wild boar strains, represented mainly by 1A biotype, were not classified into the predominant Variable-Number Tandem Repeats (VNTR)/PFGE profile and virulotype. There was a clustering tendency among VNTR/PFGE profiles of pig origin, 4/O:3, and virulence profile. Pig and human strains formed the most related group, characterized by ~80% of genetic similarity what suggest the role of pigs as a potential source of infection for the pork consumers.

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