Prevalence, Genetic Homogeneity, and Antibiotic Resistance of Pathogenic Strains Isolated from Slaughtered Pigs in Bulgaria

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2023 Apr 28

PMID 37107078

Authors

Maya Angelovska

Maya Margaritova Zaharieva

Lyudmila L Dimitrova

Tanya Dimova

Irina Gotova

Zoltan Urshev

Yana Ilieva

Mila Dobromirova Kaleva

Tanya Chan Kim

Sevda Naydenska

Zhechko Dimitrov

Hristo Najdenski

Affiliations

Soon will be listed here.

Abstract

Yersiniosis is the third most commonly reported foodborne zoonosis in the European Union. Here, we evaluated the prevalence of pathogenic among healthy pigs (as a major reservoir) in a slaughterhouse in Bulgaria. A total of 790 tonsils and feces from 601 pigs were examined. Isolation and pathogenicity characterization was carried out by the ISO 10273:2003 protocol and Polymerase Chain Reaction (PCR), detecting the gene, attachment and invasion (), heat-stable enterotoxin (), and adhesion () genes. Genetic diversity was assessed by pulsed-field gel electrophoresis (PFGE), and antimicrobial resistance by the standard disk diffusion method. Of all the pigs tested, 6.7% were positive for . All isolates belonged to bioserotype 4/O:3. , and genes were detected in all positive strains (n = 43), while the plasmid virulence plasmid (pYV) was detected in 41. High homogeneity was observed among the strains, with all strains susceptible to ceftriaxone, amikacin and ciprofloxacin, and resistant to ampicillin. In conclusion, a low prevalence of 4/O:3 was found in healthy pigs slaughtered in Bulgaria, not underestimating possible contamination of pork as a potential risk to consumer health.

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