TIMELESS Inhibits Breast Cancer Cell Invasion and Metastasis by Down-regulating the Expression of MMP9

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2021 Jan 12

PMID 33430865

Citations 5

Authors

Bowen Li

Liying Mu

Yanan Li

Kangkai Xia

Yuxi Yang

Sattout Aman

Bashir Ahmad

Shujing Li

Huijian Wu

Affiliations

Soon will be listed here.

Abstract

Breast cancer is the first killer leading to female death, and tumor metastasis is one of the important factors leading to the death of patients, but the specific mechanism of breast cancer metastasis is not very clear at present. Our study showed that overexpression of TIMELESS could significantly inhibit the invasion and metastasis of breast cancer cells ZR-75-30 and the assembly of F-actin protein. On the contrary, knockdown of TIMELESS promoted the invasion and metastasis of breast cancer cells. Further study revealed that TIMELESS overexpression decreased the mRNA and protein levels of MMP9. Furthermore, TIMELESS could interact with p65, leading to repress the association of p65 and its acetyltransferase CBP and down-regulating the acetylation level of p65, which inhibited the activation of NF-κB signal pathway. In conclusion, our research showed that TIMELESS may repress the invasion and metastasis of breast cancer cells via inhibiting the acetylation of p65, inhibiting the activation of NF-κB, thus down-regulating the expression of MMP9, and then inhibiting the invasion and metastasis of breast cancer cells.

Citing Articles

ncRNAs-mediated TIMELESS overexpression in lung adenocarcinoma correlates with reduced tumor immune cell infiltration and poor prognosis.

Gao X, Tang M, Tian S, Li J, Wei S, Hua S PLoS One. 2024; 19(1):e0296829.

PMID: 38261568 PMC: 10805305. DOI: 10.1371/journal.pone.0296829.

Six-hour time-restricted feeding inhibits lung cancer progression and reshapes circadian metabolism.

Shi D, Fang G, Chen Q, Li J, Ruan X, Lian X BMC Med. 2023; 21(1):417.

PMID: 37924048 PMC: 10625271. DOI: 10.1186/s12916-023-03131-y.

MEX3A promotes the malignant progression of ovarian cancer by regulating intron retention in TIMELESS.

Li F, Zhao C, Diao Y, Wang Z, Peng J, Yang N Cell Death Dis. 2022; 13(6):553.

PMID: 35715407 PMC: 9205863. DOI: 10.1038/s41419-022-05000-7.

Loss of circadian gene Timeless induces EMT and tumor progression in colorectal cancer via Zeb1-dependent mechanism.

Colangelo T, Carbone A, Mazzarelli F, Cuttano R, Dama E, Nittoli T Cell Death Differ. 2022; 29(8):1552-1568.

PMID: 35034102 PMC: 9345857. DOI: 10.1038/s41418-022-00935-y.

TIMELESS Promotes Tumor Progression by Enhancing Macrophages Recruitment in Ovarian Cancer.

Xing X, Gu F, Hua L, Cui X, Li D, Wu Z Front Oncol. 2021; 11:732058.

PMID: 34490127 PMC: 8417241. DOI: 10.3389/fonc.2021.732058.

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