Lectin and Binding to Carbohydrate-Functionalized Oligo(ethylene Glycol)-Based Microgels: Effect of Elastic Modulus, Crosslinker and Carbohydrate Density

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Jan 12

PMID 33430287

Authors

Fabian Schroer

Tanja J Paul

Dimitri Wilms

Torben H Saatkamp

Nicholas Jack

Janita Muller

Alexander K Strzelczyk

Stephan Schmidt

Affiliations

Soon will be listed here.

Abstract

The synthesis of carbohydrate-functionalized biocompatible poly(oligo(ethylene glycol) methacrylate microgels and the analysis of the specific binding to concanavalin A (ConA) and () is shown. By using different crosslinkers, the microgels' size, density and elastic modulus were varied. Given similar mannose (Man) functionalization degrees, the softer microgels show increased ConA uptake, possibly due to increased ConA diffusion in the less dense microgel network. Furthermore, although the microgels did not form clusters with in solution, surfaces coated with mannose-functionalized microgels are shown to bind the bacteria whereas galactose (Gal) and unfunctionalized microgels show no binding. While ConA binding depends on the overall microgels' density and Man functionalization degree, binding to microgels' surfaces appears to be largely unresponsive to changes of these parameters, indicating a rather promiscuous surface recognition and sufficiently strong anchoring to few surface-exposed Man units. Overall, these results indicate that carbohydrate-functionalized biocompatible oligo(ethylene glycol)-based microgels are able to immobilize carbohydrate binding pathogens specifically and that the binding of free lectins can be controlled by the network density.

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