LncRNA RHPN1-AS1 Serves As a Sponge for MiR-3133 Modulating the Cell Proliferation of Retinoblastoma Through JAK2

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2021 Jan 11

PMID 33426053

Citations 3

Authors

Zhao-Na Li

Ming-Xu Ge

Li-Jun Cao

Zhong-Fang Yuan

Affiliations

Soon will be listed here.

Abstract

Purpose: To investigate the effects of lncRNA RHPN1-AS1 on retinoblastoma (RB) and further explore its underlying molecular mechanisms.

Methods: The expression of RHPN1-AS1, miR-3133, (JAK2), and signal transducer and activator of transcription 3 (STAT3) was detected by qRT-PCR. CCK-8, EDU, and flow cytometry assays were conducted to assess the proliferation activity and apoptosis of RB cells. Double fluorescein and RNA immunoprecipitation assays were performed to detect the interaction between RHPN1-AS1 and miR-3133 or miR-3133 and JAK2. Western blotting was performed to detect the expression of apoptosis-related proteins.

Results: In RB cells, RHPN1-AS1 was upregulated. Silencing RHPN1-AS1 inhibited the activity of RB cells and promoted apoptosis. The expressions of proapoptotic factors (Bax and p53) were increased, while antiapoptotic factors (Bcl-2 and Survivin) were suppressed in siRHPN1-AS1 groups. Furthermore, we predicted and verified that RHPN1-AS1 regulated RB progression by targeting miR-3133/JAK2. In addition, siRHPN1-AS1 also inhibited oncogene STAT3 protein expression.

Conclusion: lncRNA RHPN1-AS1 served as a sponge for miR-3133 to counteract miR-3133-mediated JAK2/STAT3 suppression, indicating that the lncRNA RHPN1-AS1 may be a potential therapeutic target for the treatment of RB.

Citing Articles

miR-3133 inhibits gastrointestinal cancer progression through activation of Hippo and p53 signalling pathways via multi-targets.

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miR-3133 is an unfavorable prognosis factor and tumor suppressor in colon cancer.

Gao H, Zhang C Funct Integr Genomics. 2023; 23(2):132.

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Role of non-coding RNAs and exosomal non-coding RNAs in retinoblastoma progression.

Ahangar Davoodi N, Najafi S, Ghale-Noie Z, Piranviseh A, Mollazadeh S, Ahmadi Asouri S Front Cell Dev Biol. 2023; 10:1065837.

PMID: 36619866 PMC: 9816416. DOI: 10.3389/fcell.2022.1065837.

References

Zhu P, Li Y, Li P, Zhang Y, Wang X . c-Myc induced the regulation of long non-coding RNA RHPN1-AS1 on breast cancer cell proliferation via inhibiting P53. Mol Genet Genomics. 2019; 294(5):1219-1229. DOI: 10.1007/s00438-019-01572-w. View

Chen X . Expression of microRNA-3133 correlates with the prognosis in patients with clear cell renal cell carcinoma. Medicine (Baltimore). 2019; 98(24):e16008. PMC: 6587602. DOI: 10.1097/MD.0000000000016008. View

Mendoza P, Grossniklaus H . Therapeutic Options for Retinoblastoma. Cancer Control. 2016; 23(2):99-109. DOI: 10.1177/107327481602300203. View

Lu L, Yu X, Zhang L, Ding X, Pan H, Wen X . The Long Non-Coding RNA RHPN1-AS1 Promotes Uveal Melanoma Progression. Int J Mol Sci. 2017; 18(1). PMC: 5297855. DOI: 10.3390/ijms18010226. View

Duan H, Li X, Chen Y, Wang Y, Li Z . LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299-3p/FGF2 axis. Life Sci. 2019; 239:116856. DOI: 10.1016/j.lfs.2019.116856. View

Uszczynska-Ratajczak B, Lagarde J, Frankish A, Guigo R, Johnson R . Towards a complete map of the human long non-coding RNA transcriptome. Nat Rev Genet. 2018; 19(9):535-548. PMC: 6451964. DOI: 10.1038/s41576-018-0017-y. View

Adams B, Parsons C, Walker L, Zhang W, Slack F . Targeting noncoding RNAs in disease. J Clin Invest. 2017; 127(3):761-771. PMC: 5330746. DOI: 10.1172/JCI84424. View

Anastasiadou E, Jacob L, Slack F . Non-coding RNA networks in cancer. Nat Rev Cancer. 2017; 18(1):5-18. PMC: 6337726. DOI: 10.1038/nrc.2017.99. View

Kivela T . The epidemiological challenge of the most frequent eye cancer: retinoblastoma, an issue of birth and death. Br J Ophthalmol. 2009; 93(9):1129-31. DOI: 10.1136/bjo.2008.150292. View

10.

Hosseini E, Meryet-Figuiere M, Sabzalipoor H, Haddad Kashani H, Nikzad H, Asemi Z . Dysregulated expression of long noncoding RNAs in gynecologic cancers. Mol Cancer. 2017; 16(1):107. PMC: 5480155. DOI: 10.1186/s12943-017-0671-2. View

11.

Narang S, Mashayekhi A, Rudich D, Shields C . Predictors of long-term visual outcome after chemoreduction for management of intraocular retinoblastoma. Clin Exp Ophthalmol. 2012; 40(7):736-42. DOI: 10.1111/j.1442-9071.2012.02757.x. View

12.

Dimaras H, Dimba E, Gallie B . Challenging the global retinoblastoma survival disparity through a collaborative research effort. Br J Ophthalmol. 2010; 94(11):1415-6. DOI: 10.1136/bjo.2009.174136. View

13.

Piao X, Zou Y, Sui X, Liu B, Meng F, Li S . Hydrostatin-SN10 Ameliorates Pancreatitis-Induced Lung Injury by Affecting IL-6-Induced JAK2/STAT3-Associated Inflammation and Oxidative Stress. Oxid Med Cell Longev. 2019; 2019:9659757. PMC: 6885838. DOI: 10.1155/2019/9659757. View

14.

AlAli A, Kletke S, Gallie B, Lam W . Retinoblastoma for Pediatric Ophthalmologists. Asia Pac J Ophthalmol (Phila). 2018; 7(3):160-168. DOI: 10.22608/APO.201870. View

15.

Emeagi P, Maenhout S, Dang N, Heirman C, Thielemans K, Breckpot K . Downregulation of Stat3 in melanoma: reprogramming the immune microenvironment as an anticancer therapeutic strategy. Gene Ther. 2013; 20(11):1085-92. DOI: 10.1038/gt.2013.35. View

16.

Liu L, Nam S, Tian Y, Yang F, Wu J, Wang Y . 6-Bromoindirubin-3'-oxime inhibits JAK/STAT3 signaling and induces apoptosis of human melanoma cells. Cancer Res. 2011; 71(11):3972-9. PMC: 3107399. DOI: 10.1158/0008-5472.CAN-10-3852. View

17.

Chen L, Yao H, Wang K, Liu X . Long Non-Coding RNA MALAT1 Regulates ZEB1 Expression by Sponging miR-143-3p and Promotes Hepatocellular Carcinoma Progression. J Cell Biochem. 2017; 118(12):4836-4843. DOI: 10.1002/jcb.26158. View

18.

Niu G, Bowman T, Huang M, Shivers S, Reintgen D, Daud A . Roles of activated Src and Stat3 signaling in melanoma tumor cell growth. Oncogene. 2002; 21(46):7001-10. DOI: 10.1038/sj.onc.1205859. View

19.

Jarskog L, Selinger E, Lieberman J, Gilmore J . Apoptotic proteins in the temporal cortex in schizophrenia: high Bax/Bcl-2 ratio without caspase-3 activation. Am J Psychiatry. 2004; 161(1):109-15. DOI: 10.1176/appi.ajp.161.1.109. View

20.

Qian K, Liu G, Tang Z, Hu Y, Fang Y, Chen Z . The long non-coding RNA NEAT1 interacted with miR-101 modulates breast cancer growth by targeting EZH2. Arch Biochem Biophys. 2016; 615:1-9. DOI: 10.1016/j.abb.2016.12.011. View