» Articles » PMID: 33419049

Spinochrome Identification and Quantification in Pacific Sea Urchin Shells, Coelomic Fluid and Eggs Using HPLC-DAD-MS

Overview
Journal Mar Drugs
Publisher MDPI
Specialties Biology
Pharmacology
Date 2021 Jan 9
PMID 33419049
Citations 6
Authors
Affiliations
Soon will be listed here.
Abstract

The high-performance liquid chromatography method coupled with diode array and mass spectrometric detector (HPLC-DAD-MS) method for quinonoid pigment identification and quantification in sea urchin samples was developed and validated. The composition and quantitative ratio of the quinonoid pigments of the shells of 16 species of sea urchins, collected in the temperate (Sea of Japan) and tropical (South-China Sea) climatic zones of the Pacific Ocean over several years, were studied. The compositions of the quinonoid pigments of sea urchins , , and were studied for the first time. A study of the composition of the quinonoid pigments of the coelomic fluid of ten species of sea urchins was conducted. The composition of quinonoid pigments of jelly-like egg membrane, of developing embryos and pluteus, was reported for the first time. In the case of , we have shown that the compositions of pigment granules of the shell epidermis, coelomic fluid, egg membrane, developing embryos and pluteus are different, which should enable a fuller understanding of the functions of pigments at different stages of life.

Citing Articles

Phanogracilins A-C, New Bibenzochromenones of Crinoid (Hartlaub, 1890).

Vasileva E, Berdyshev D, Mishchenko N, Gerasimenko A, Menchinskaya E, Pislyagin E Biomolecules. 2024; 14(2).

PMID: 38397388 PMC: 10887160. DOI: 10.3390/biom14020151.


Dimeric (Poly)Hydroxynaphthazarins, Metabolites of Echinoderms and Lichens: The History of the Synthesis and Structure Elucidation.

Pelageev D, Borisova K, Anufriev V Mar Drugs. 2023; 21(7).

PMID: 37504938 PMC: 10381475. DOI: 10.3390/md21070407.


Development of Novel Pharmaceutical Forms of the Marine Bioactive Pigment Echinochrome A Enabling Alternative Routes of Administration.

Kikionis S, Papakyriakopoulou P, Mavrogiorgis P, Vasileva E, Mishchenko N, Fedoreyev S Mar Drugs. 2023; 21(4).

PMID: 37103389 PMC: 10147083. DOI: 10.3390/md21040250.


Echinochrome A Prevents Diabetic Nephropathy by Inhibiting the PKC-Iota Pathway and Enhancing Renal Mitochondrial Function in db/db Mice.

Pham T, Nguyen T, Yun H, Vasileva E, Mishchenko N, Fedoreyev S Mar Drugs. 2023; 21(4).

PMID: 37103361 PMC: 10142928. DOI: 10.3390/md21040222.


Quinoid Pigments of Sea Urchins and : Biological Activity and Potential Applications.

Ageenko N, Kiselev K, Odintsova N Mar Drugs. 2022; 20(10).

PMID: 36286435 PMC: 9605347. DOI: 10.3390/md20100611.


References
1.
Brasseur L, Hennebert E, Fievez L, Caulier G, Bureau F, Tafforeau L . The Roles of Spinochromes in Four Shallow Water Tropical Sea Urchins and Their Potential as Bioactive Pharmacological Agents. Mar Drugs. 2017; 15(6). PMC: 5484129. DOI: 10.3390/md15060179. View

2.
Lebedev A, Ivanova M, Levitsky D . Iron chelators and free radical scavengers in naturally occurring polyhydroxylated 1,4-naphthoquinones. Hemoglobin. 2008; 32(1-2):165-79. DOI: 10.1080/03630260701700017. View

3.
Vasileva E, Mishchenko N, Zadorozhny P, Fedoreyev S . New Aminonaphthoquinone from the Sea Urchins Strongylocentrotus pallidus and Mesocentrotus nudus. Nat Prod Commun. 2016; 11(6):821-4. View

4.
NISHIBORI K . Isolation of echinochrome A from the spines of the sea urchin, Stomopneustes variolaris (Lamarck). Nature. 1961; 192:1293-4. DOI: 10.1038/1921293a0. View

5.
Vasileva E, Mishchenko N, Fedoreyev S . Diversity of Polyhydroxynaphthoquinone Pigments in North Pacific Sea Urchins. Chem Biodivers. 2017; 14(9). DOI: 10.1002/cbdv.201700182. View