Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy

Overview

Journal Diagnostics (Basel)

Specialty Radiology

Date 2021 Jan 9

PMID 33418979

Citations 10

Authors

Vittoria Mattioni Marchetti

Ibrahim Bitar

Mario Sarti

Elena Fogato

Erika Scaltriti

Chiara Bracchi

Jaroslav Hrabak

Stefano Pongolini

Roberta Migliavacca

Affiliations

Soon will be listed here.

Abstract

Background: the co-production of carbapenemases and -genes represents a worrisome event in the treatment of infections. The aim of the study was to characterize the genomic features of two clinical complex (ECC) isolates, co-producing VIM and MCR enzymes, in Italy.

Methods: species identification and antibiotic susceptibility profiling were performed using MALDI-TOF and broth microdilution methods, respectively. Transferability of the and - type genes was verified through conjugation experiment. Extracted DNA was sequenced using long reads sequencing technology on the Sequel I platform (PacBio).

Results: the first isolate showed clinical resistance against ertapenem yet was colistin susceptible (EUCAST 2020 breakpoints). The gene was harbored on a conjugative IncHI2 plasmid, while the determinant was harbored on a conjugative IncN plasmid. The second isolate, resistant to both carbapenems and colistin, harbored: gene and its two component regulatory genes for increased expression on the chromosome, on non-conjugative (yet co-transferable) ColE plasmid, and on a non-conjugative IncA plasmid.

Conclusions: to our knowledge, this is the first report of co-production of VIM and MCR in ECC isolates in Italy.

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