Bacterial Metabolosomes: New Insights into Their Structure and Bioengineering

Overview

Journal Microb Biotechnol

Specialties Biotechnology
Microbiology

Date 2021 Jan 6

PMID 33404191

Citations 14

Authors

Lu-Ning Liu

Affiliations

Soon will be listed here.

Abstract

Bacterial metabolosomes have been discovered for over 25 years. They play essential roles in bacterial metabolism and pathogenesis. In this crystal ball paper, I will discuss the recent advances in the fundamental understanding and synthetic engineering of bacterial metabolosomes.

Citing Articles

Molecular principles of the assembly and construction of a carboxysome shell.

Wang P, Li J, Li T, Li K, Ng P, Wang S Sci Adv. 2024; 10(48):eadr4227.

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Rubisco packaging and stoichiometric composition of a native β-carboxysome.

Sun Y, Sheng Y, Ni T, Ge X, Sarsby J, Brownridge P bioRxiv. 2024; .

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Nanoengineering Carboxysome Shells for Protein Cages with Programmable Cargo Targeting.

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Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly.

Ni T, Jiang Q, Ng P, Shen J, Dou H, Zhu Y Nat Commun. 2023; 14(1):5512.

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Single-particle cryo-EM analysis of the shell architecture and internal organization of an intact α-carboxysome.

Evans S, Al-Hazeem M, Mann D, Smetacek N, Beavil A, Sun Y Structure. 2023; 31(6):677-688.e4.

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References

Lee M, Mantell J, Hodgson L, Alibhai D, Fletcher J, Brown I . Engineered synthetic scaffolds for organizing proteins within the bacterial cytoplasm. Nat Chem Biol. 2017; 14(2):142-147. DOI: 10.1038/nchembio.2535. View

Sun Y, Casella S, Fang Y, Huang F, Faulkner M, Barrett S . Light Modulates the Biosynthesis and Organization of Cyanobacterial Carbon Fixation Machinery through Photosynthetic Electron Flow. Plant Physiol. 2016; 171(1):530-41. PMC: 4854705. DOI: 10.1104/pp.16.00107. View

Juodeikis R, Lee M, Mayer M, Mantell J, Brown I, Verkade P . Effect of metabolosome encapsulation peptides on enzyme activity, coaggregation, incorporation, and bacterial microcompartment formation. Microbiologyopen. 2020; 9(5):e1010. PMC: 7221423. DOI: 10.1002/mbo3.1010. View

Liang M, Frank S, Lunsdorf H, Warren M, Prentice M . Bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation in E. coli. Biotechnol J. 2017; 12(3). DOI: 10.1002/biot.201600415. View

Axen S, Erbilgin O, Kerfeld C . A taxonomy of bacterial microcompartment loci constructed by a novel scoring method. PLoS Comput Biol. 2014; 10(10):e1003898. PMC: 4207490. DOI: 10.1371/journal.pcbi.1003898. View

Sutter M, Greber B, Aussignargues C, Kerfeld C . Assembly principles and structure of a 6.5-MDa bacterial microcompartment shell. Science. 2017; 356(6344):1293-1297. PMC: 5873307. DOI: 10.1126/science.aan3289. View

Lee M, Brown I, Juodeikis R, Frank S, Warren M . Employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in Escherichia coli. Metab Eng. 2016; 36:48-56. PMC: 4909751. DOI: 10.1016/j.ymben.2016.02.007. View

Dai W, Chen M, Myers C, Ludtke S, Pettitt B, King J . Visualizing Individual RuBisCO and Its Assembly into Carboxysomes in Marine Cyanobacteria by Cryo-Electron Tomography. J Mol Biol. 2018; 430(21):4156-4167. PMC: 6252266. DOI: 10.1016/j.jmb.2018.08.013. View

Yeates T, Crowley C, Tanaka S . Bacterial microcompartment organelles: protein shell structure and evolution. Annu Rev Biophys. 2010; 39:185-205. PMC: 3272493. DOI: 10.1146/annurev.biophys.093008.131418. View

10.

Sutter M, Faulkner M, Aussignargues C, Paasch B, Barrett S, Kerfeld C . Visualization of Bacterial Microcompartment Facet Assembly Using High-Speed Atomic Force Microscopy. Nano Lett. 2015; 16(3):1590-5. PMC: 4789755. DOI: 10.1021/acs.nanolett.5b04259. View

11.

Lee M, Mantell J, Brown I, Fletcher J, Verkade P, Pickersgill R . De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments. Nat Commun. 2018; 9(1):3413. PMC: 6109187. DOI: 10.1038/s41467-018-05922-x. View

12.

Huang F, Kong W, Sun Y, Chen T, Dykes G, Jiang Y . Rubisco accumulation factor 1 (Raf1) plays essential roles in mediating Rubisco assembly and carboxysome biogenesis. Proc Natl Acad Sci U S A. 2020; 117(29):17418-17428. PMC: 7382273. DOI: 10.1073/pnas.2007990117. View

13.

Gibson T, Lamond A . Metabolic complexity in the RNA world and implications for the origin of protein synthesis. J Mol Evol. 1990; 30(1):7-15. DOI: 10.1007/BF02102448. View

14.

Iancu C, Morris D, Dou Z, Heinhorst S, Cannon G, Jensen G . Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells. J Mol Biol. 2009; 396(1):105-17. PMC: 2853366. DOI: 10.1016/j.jmb.2009.11.019. View

15.

Ravcheev D, Moussu L, Smajic S, Thiele I . Comparative Genomic Analysis Reveals Novel Microcompartment-Associated Metabolic Pathways in the Human Gut Microbiome. Front Genet. 2019; 10:636. PMC: 6620236. DOI: 10.3389/fgene.2019.00636. View

16.

Brinsmade S, Paldon T, Escalante-Semerena J . Minimal functions and physiological conditions required for growth of salmonella enterica on ethanolamine in the absence of the metabolosome. J Bacteriol. 2005; 187(23):8039-46. PMC: 1291257. DOI: 10.1128/JB.187.23.8039-8046.2005. View

17.

Cameron J, Wilson S, Bernstein S, Kerfeld C . Biogenesis of a bacterial organelle: the carboxysome assembly pathway. Cell. 2013; 155(5):1131-40. DOI: 10.1016/j.cell.2013.10.044. View

18.

Greber B, Sutter M, Kerfeld C . The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly. Structure. 2019; 27(5):749-763.e4. PMC: 6506404. DOI: 10.1016/j.str.2019.01.017. View

19.

Zhao L, Huokko T, Wilson S, Simpson D, Wang Q, Ruban A . Structural variability, coordination and adaptation of a native photosynthetic machinery. Nat Plants. 2020; 6(7):869-882. DOI: 10.1038/s41477-020-0694-3. View

20.

Li T, Jiang Q, Huang J, Aitchison C, Huang F, Yang M . Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nat Commun. 2020; 11(1):5448. PMC: 7595155. DOI: 10.1038/s41467-020-19280-0. View