Molecular Survey of Selected Viral Pathogens in Wild Leopard Cats (Prionailurus Bengalensis) in Taiwan with an Emphasis on the Spatial and Temporal Dynamics of Carnivore Protoparvovirus 1

Overview

Journal Arch Virol

Specialty Microbiology

Date 2021 Jan 3

PMID 33389172

Citations 2

Authors

Chen-Chih Chen

Ai-Mei Chang

Wan-Jhen Chen

Po-Jen Chang

Yu-Ching Lai

Hsu-Hsun Lee

Affiliations

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Abstract

The leopard cat (Prionailurus bengalensis) was listed as an endangered species under the Wildlife Conservation Act in Taiwan in 2009. However, no study has evaluated the possible direct or indirect effects of pathogens on the Taiwanese leopard cat population. Here, we targeted viral pathogens, including carnivore protoparvovirus 1 (genus Protoparvovirus), feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), coronaviruses (CoVs), and canine distemper virus (CDV), through molecular screening. The spatial and temporal dynamics of the target pathogens were evaluated. Through sequencing and phylogenetic analysis, we clarified the phylogenetic relationship of viral pathogens isolated from leopard cats and domestic carnivores. Samples from 23 live-trapped leopard cats and 29 that were found dead were collected from 2015 to 2019 in Miaoli County in northwestern Taiwan. Protoparvoviruses and CoVs were detected in leopard cats, and their prevalence (95% confidence interval) was 63.5% (50.4%-76.6%) and 8.8% (0%-18.4%), respectively. Most of the protoparvovirus sequences amplified from Taiwanese leopard cats and domestic carnivores were identical. All of the CoV sequences amplified from leopard cats were identified as feline CoV. No spatial or temporal aggregation of protoparvovirus infection in leopard cats was found in the sampling area, indicating a wide distribution of protoparvoviruses in the leopard cat habitat. We consider sympatric domestic carnivores to be the probable primary reservoir for the identified pathogens. We strongly recommend management of protoparvoviruses and feline CoV in the leopard cat habitat, particularly vaccination programs and population control measures for free-roaming dogs and cats.

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References

Chen C, Chang A, Wada T, Chen M, Tu Y . Distribution of Carnivore protoparvovirus 1 in free-living leopard cats (Prionailurus bengalensis chinensis) and its association with domestic carnivores in Taiwan. PLoS One. 2019; 14(9):e0221990. PMC: 6719846. DOI: 10.1371/journal.pone.0221990. View

Daszak P, Cunningham A, Hyatt A . Emerging infectious diseases of wildlife--threats to biodiversity and human health. Science. 2000; 287(5452):443-9. DOI: 10.1126/science.287.5452.443. View

Hayama S, Yamamoto H, Nakanishi S, Hiyama T, Murayama A, Mori H . Risk analysis of feline immunodeficiency virus infection in Tsushima leopard cats (Prionailurus bengalensis euptilurus) and domestic cats using a geographic information system. J Vet Med Sci. 2010; 72(9):1113-8. DOI: 10.1292/jvms.09-0502. View

Nakamura K, Ikeda Y, Miyazawa T, Nguyen N, Duong D, Le K . Comparison of prevalence of feline herpesvirus type 1, calicivirus and parvovirus infections in domestic and leopard cats in Vietnam. J Vet Med Sci. 2000; 61(12):1313-5. DOI: 10.1292/jvms.61.1313. View

Ikeda Y, Miyazawa T, Nakamura K, Naito R, Inoshima Y, Tung K . Serosurvey for selected virus infections of wild carnivores in Taiwan and Vietnam. J Wildl Dis. 1999; 35(3):578-81. DOI: 10.7589/0090-3558-35.3.578. View

Hwang J, Oh D, Lee H, Chun M . Anaplasma sp. and hemoplasma infection in leopard cats (Prionailurus bengalensis euptilurus) from Korea. J Vet Sci. 2015; 16(3):385-8. PMC: 4588026. DOI: 10.4142/jvs.2015.16.3.385. View

Tateno M, Nishio T, Sakuma M, Nakanishi N, Izawa M, Asari Y . Molecular epidemiologic survey of Bartonella, Ehrlichia, and Anaplasma infections in Japanese Iriomote and Tsushima leopard cats. J Wildl Dis. 2013; 49(3):646-52. DOI: 10.7589/2012-07-194. View

Hirata M, Tateno M, Sakuma M, Nakanishi N, Izawa M, Asari Y . An epidemiological survey of hemoplasma infection in Iriomote cats (Prionailurus bengalensis iriomotensis). J Vet Med Sci. 2012; 74(12):1531-7. DOI: 10.1292/jvms.12-0094. View

Kubo M, Miyoshi N, Yasuda N . Hepatozoonosis in two species of Japanese wild cat. J Vet Med Sci. 2006; 68(8):833-7. DOI: 10.1292/jvms.68.833. View

10.

Sakuma M, Nishio T, Nakanishi N, Izawa M, Asari Y, Okamura M . A case of Iriomote cat (Prionailurus bengalensis iriomotensis) with Hepatozoon felis parasitemia. J Vet Med Sci. 2011; 73(10):1381-4. DOI: 10.1292/jvms.11-0210. View

11.

Tateno M, Nishio T, Matsuo T, Sakuma M, Nakanishi N, Izawa M . Epidemiological survey of tick-borne protozoal infection in iriomote cats and tsushima leopard cats in Japan. J Vet Med Sci. 2013; 75(7):985-9. DOI: 10.1292/jvms.13-0015. View

12.

Anthony S, Epstein J, A Murray K, Navarrete-Macias I, Zambrana-Torrelio C, Solovyov A . A strategy to estimate unknown viral diversity in mammals. mBio. 2013; 4(5):e00598-13. PMC: 3760253. DOI: 10.1128/mBio.00598-13. View

13.

Steinel A, Munson L, van Vuuren M, Truyen U . Genetic characterization of feline parvovirus sequences from various carnivores. J Gen Virol. 2000; 81(Pt 2):345-50. DOI: 10.1099/0022-1317-81-2-345. View

14.

Miyazawa T, Jarrett O . Feline leukaemia virus proviral DNA detected by polymerase chain reaction in antigenaemic but non-viraemic ('discordant') cats. Arch Virol. 1997; 142(2):323-32. DOI: 10.1007/s007050050079. View

15.

Poon L, Chu D, Chan K, Wong O, Ellis T, Leung Y . Identification of a novel coronavirus in bats. J Virol. 2005; 79(4):2001-9. PMC: 546586. DOI: 10.1128/JVI.79.4.2001-2009.2005. View

16.

Tong S, Chern S, Li Y, Pallansch M, Anderson L . Sensitive and broadly reactive reverse transcription-PCR assays to detect novel paramyxoviruses. J Clin Microbiol. 2008; 46(8):2652-8. PMC: 2519498. DOI: 10.1128/JCM.00192-08. View

17.

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View

18.

Hamm R, Gottesmann P . [Lipoamide dehydrogenase, citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase in skeletal muscle. VIII. The influence of temperature and rate of freezing of bovine muscle on the activity and subcellular distribution of the enzymes in the thawed...]. Z Lebensm Unters Forsch. 1985; 181(3):210-6. DOI: 10.1007/BF02425580. View

19.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S . MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-9. PMC: 3203626. DOI: 10.1093/molbev/msr121. View

20.

Duarte M, Henriques A, Barros S, Fagulha T, Mendonca P, Carvalho P . Snapshot of viral infections in wild carnivores reveals ubiquity of parvovirus and susceptibility of Egyptian mongoose to feline panleukopenia virus. PLoS One. 2013; 8(3):e59399. PMC: 3603882. DOI: 10.1371/journal.pone.0059399. View