MiRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2020 Dec 31

PMID 33381563

Citations 8

Authors

Yi Sun

Xin Wang

Guanghua Chen

Chengchao Song

Xinnan Ma

Yutuo Fu

Chao Feng

Jinglong Yan

Affiliations

Soon will be listed here.

Abstract

Osteoporosis (OP) is a common bone metabolic disease, the process of which is fundamentally irreversible. Therefore, the investigation into osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) will provide more clues for OP treatment. In the present study, we found that microRNA-187-5p (miR-187-5p) played a key role on osteoblastic differentiation, which was significantly upregulated during osteogenic differentiation of BMSCs in mice. Moreover, overexpression of miR-187-5p suppressed osteoblastic differentiation of BMSCs through increasing alkaline phosphatase (ALP), matrix mineralization, and levels of Osterix (OSX), and osteopontin (OPN) as well as runt-related transcription factor 2 (Runx2) . The results indicated that the upregulation of miR-187-5p enhanced the efficacy of new bone formation in the heterotopic bone formation assay. Luciferase reporter assay and western blot analysis revealed that miR-187-5p was involved in osteogenesis by targeting intracellular adhesion molecule 1 (ICAM-1). Furthermore, ICAM-1 silence inhibited osteoblastic differentiation of BMSCs. Taken together, our results suggested for the first time that miR-187-5p may promote osteogenesis by targeting ICAM-1, and provided a possible therapeutic target for bone metabolic diseases.

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References

Elsafadi M, Manikandan M, Alajez N, Hamam R, Dawud R, Aldahmash A . MicroRNA-4739 regulates osteogenic and adipocytic differentiation of immortalized human bone marrow stromal cells via targeting LRP3. Stem Cell Res. 2017; 20:94-104. DOI: 10.1016/j.scr.2017.03.001. View

Lin M, Xue X, Liang S, Li Y, Lv Y, He L . MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6. Oncotarget. 2017; 8(38):62914-62926. PMC: 5609891. DOI: 10.18632/oncotarget.17516. View

Long H, Sun B, Cheng L, Zhao S, Zhu Y, Zhao R . miR-139-5p Represses BMSC Osteogenesis via Targeting Wnt/β-Catenin Signaling Pathway. DNA Cell Biol. 2017; 36(8):715-724. DOI: 10.1089/dna.2017.3657. View

Cai Y, Ruan J, Yao X, Zhao L, Wang B . MicroRNA-187 modulates epithelial-mesenchymal transition by targeting PTRF in non-small cell lung cancer. Oncol Rep. 2017; 37(5):2787-2794. DOI: 10.3892/or.2017.5548. View

Lou Y, Liu L, Zhan L, Wang X, Fan H . miR-187-5p Regulates Cell Growth and Apoptosis in Acute Lymphoblastic Leukemia via DKK2. Oncol Res. 2016; 24(2):89-97. PMC: 7838722. DOI: 10.3727/096504016X14597766487753. View

Wang J, Zhao Z, Luo S, Fan Y . [Expression of osteoclast differentiation factor and intercellular adhesion molecule-1 of bone marrow mesenchymal stem cells enhanced with osteogenic differentiation]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2005; 23(3):240-3. View

Berry S, Shi S, Kiel D . Considering the Risks and Benefits of Osteoporosis Treatment in Older Adults. JAMA Intern Med. 2019; 179(8):1103-1104. DOI: 10.1001/jamainternmed.2019.0688. View

Deng L, Hu G, Jin L, Wang C, Niu H . Involvement of microRNA-23b in TNF-α-reduced BMSC osteogenic differentiation via targeting runx2. J Bone Miner Metab. 2017; 36(6):648-660. DOI: 10.1007/s00774-017-0886-8. View

Li Y, Feng C, Gao M, Jin M, Liu T, Yuan Y . MicroRNA-92b-5p modulates melatonin-mediated osteogenic differentiation of bone marrow mesenchymal stem cells by targeting ICAM-1. J Cell Mol Med. 2019; 23(9):6140-6153. PMC: 6714169. DOI: 10.1111/jcmm.14490. View

10.

Blumstein T, Benyamini Y, Farhi A, Boyko V, Lerner-Geva L . Knowledge of risk factors and prevention of osteoporosis: the Israeli women's health at midlife study. Arch Osteoporos. 2018; 13(1):70. DOI: 10.1007/s11657-018-0474-0. View

11.

Xu F, Zhu H, Li X, Yang F, Chen J, Tang B . Intercellular adhesion molecule-1 inhibits osteogenic differentiation of mesenchymal stem cells and impairs bio-scaffold-mediated bone regeneration in vivo. Tissue Eng Part A. 2014; 20(19-20):2768-82. PMC: 4195481. DOI: 10.1089/ten.TEA.2014.0007. View

12.

Xiao Y, Zhao Q, Du B, Chen H, Zhou D . MicroRNA-187 Inhibits Growth and Metastasis of Osteosarcoma by Downregulating S100A4. Cancer Invest. 2018; 36(1):1-9. DOI: 10.1080/07357907.2017.1415348. View

13.

Chang M, Lin H, Fu H, Wang B, Han G, Fan M . MicroRNA-195-5p Regulates Osteogenic Differentiation of Periodontal Ligament Cells Under Mechanical Loading. J Cell Physiol. 2017; 232(12):3762-3774. DOI: 10.1002/jcp.25856. View

14.

Mao M, Wu Z, Chen J . MicroRNA-187-5p suppresses cancer cell progression in non-small cell lung cancer (NSCLC) through down-regulation of CYP1B1. Biochem Biophys Res Commun. 2016; 478(2):649-55. DOI: 10.1016/j.bbrc.2016.08.001. View

15.

Hu H, Zhao C, Zhang P, Liu Y, Jiang Y, Wu E . miR-26b modulates OA induced BMSC osteogenesis through regulating GSK3β/β-catenin pathway. Exp Mol Pathol. 2019; 107:158-164. DOI: 10.1016/j.yexmp.2019.02.003. View

16.

Chen J, Yang Y, Dong X, Dong X, Wang Y, Huang X . [MicroRNA-20a Promotes Osteogenic Differentiation of C3H/10T1/2 Cells through Regulating CKIP-1 Expression]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017; 25(1):214-220. DOI: 10.7534/j.issn.1009-2137.2017.01.038. View

17.

Ren G, Roberts A, Shi Y . Adhesion molecules: key players in Mesenchymal stem cell-mediated immunosuppression. Cell Adh Migr. 2010; 5(1):20-2. PMC: 3038091. DOI: 10.4161/cam.5.1.13491. View

18.

Ren G, Zhao X, Zhang L, Zhang J, LHuillier A, Ling W . Inflammatory cytokine-induced intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in mesenchymal stem cells are critical for immunosuppression. J Immunol. 2010; 184(5):2321-8. PMC: 2881946. DOI: 10.4049/jimmunol.0902023. View

19.

Xu A, Yang Y, Shao Y, Wu M, Sun Y . Inhibiting effect of microRNA-187-3p on osteogenic differentiation of osteoblast precursor cells by suppressing cannabinoid receptor type 2. Differentiation. 2019; 109:9-15. DOI: 10.1016/j.diff.2019.07.002. View

20.

van Wijnen A, van de Peppel J, van Leeuwen J, Lian J, Stein G, Westendorf J . MicroRNA functions in osteogenesis and dysfunctions in osteoporosis. Curr Osteoporos Rep. 2013; 11(2):72-82. PMC: 3678273. DOI: 10.1007/s11914-013-0143-6. View