The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition Between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2020 Dec 31

PMID 33381190

Citations 8

Authors

Feng Qin

Jiuzuo Huang

Wenchao Zhang

Mingzi Zhang

Zhenjiang Li

Loubin Si

Xiao Long

Xiaojun Wang

Affiliations

Soon will be listed here.

Abstract

Background: Human dermal fibroblasts (HDFs) are the primary cells in skin and are associated with UVB-induced skin photoaging. Adipose-derived stem cells (ASCs) have been proposed as a treatment for skin aging. The goal of this study was to investigate paracrine mechanisms by which ASCs repair HDFs damage from UVB exposure.

Methods: ASCs were cocultured with UVB-irradiated and nonirradiated HDFs. We compared HDF senescence, proliferation, migration, oxidative stress, and cytokine expression. In a nude mouse UVB-induced photoaging model, ASCs were injected subcutaneously, and skin samples were collected weekly between postoperative weeks 3 through 7. Histological analysis, PCR, ELISA, and immunohistochemistry were used to analyze the effect of ASCs.

Results: Compared with UVB-irradiated HDFs, nonirradiated HDFs showed higher proliferation and migration, reduced apoptosis, and fewer senescent cells when cocultured with ASCs. The expression of extracellular matrix-related cytokines was also regulated by ASCs. In addition, ASCs effectively reversed UVB-induced skin photoaging in vivo. We propose that ASCs more robustly coordinate healthy HDFs than UVB-damaged HDFs to repair aging skin.

Conclusions: ASCs improved the function of both UVB-damaged and healthy HDFs through paracrine effects. However, the impact of ASCs on healthy HDFs was greater than UVB-damaged HDFs. These findings help to elucidate the underlying mechanisms of the skin rejuvenation effect of ASCs.

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