An Electrokinetically-Driven Microchip for Rapid Entrapment and Detection of Nanovesicles

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2020 Dec 30

PMID 33374467

Citations 9

Authors

Leilei Shi

Leyla Esfandiari

Affiliations

Soon will be listed here.

Abstract

Electrical Impedance Spectroscopy (EIS) has been widely used as a label-free and rapid characterization method for the analysis of cells in clinical research. However, the related work on exosomes (40-150 nm) and the particles of similar size has not yet been reported. In this study, we developed a new Lab-on-a-Chip (LOC) device to rapidly entrap a cluster of sub-micron particles, including polystyrene beads, liposomes, and small extracellular vesicles (exosomes), utilizing an insulator-based dielectrophoresis (iDEP) scheme followed by measuring their impedance utilizing an integrated electrical impedance sensor. This technique provides a label-free, fast, and non-invasive tool for the detection of bionanoparticles based on their unique dielectric properties. In the future, this device could potentially be applied to the characterization of pathogenic exosomes and viruses of similar size, and thus, be evolved as a powerful tool for early disease diagnosis and prognosis.

Citing Articles

Strategies for targeted gene delivery using lipid nanoparticles and cell-derived nanovesicles.

Lee D, Amirthalingam S, Lee C, Rajendran A, Ahn Y, Hwang N Nanoscale Adv. 2023; 5(15):3834-3856.

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A drug delivery system constructed by a fusion peptide capturing exosomes targets to titanium implants accurately resulting the enhancement of osseointegration peri-implant.

Li X, Liu Z, Xu S, Ma X, Zhao Z, Hu H Biomater Res. 2022; 26(1):89.

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A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes.

Shi L, Esfandiari L PLoS One. 2022; 17(7):e0270844.

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A Digital Microfluidic Device Integrated with Electrochemical Impedance Spectroscopy for Cell-Based Immunoassay.

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A Label-Free Electrical Impedance Spectroscopy for Detection of Clusters of Extracellular Vesicles Based on Their Unique Dielectric Properties.

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