Controllable Assembly of Upconversion Nanoparticles Enhanced Tumor Cell Penetration and Killing Efficiency

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2020 Dec 21

PMID 33344124

Citations 13

Authors

Zhen Zhang

Juwita Norasmara Rahmat

Ratha Mahendran

Yong Zhang

Affiliations

Soon will be listed here.

Abstract

The use of upconversion nanoparticles (UCNPs) for treating deep-seated cancers and large tumors has recently been gaining momentum. Conventional approaches for loading photosensitizers (PS) to UCNPs using noncovalent physical adsorption and covalent conjugation had been previously described. However, these methods are time-consuming and require extra modification steps. Incorporating PS loading during the controlled UCNPs assembly process is seldom reported. In this study, an amphiphilic copolymer, poly(styrene--maleic anhydride), is used to instruct UCNPs assembly formations into well-controlled UCNPs clusters of various sizes, and the gap zones formed between individual UCNPs can be used to encapsulate PS. This nanostructure production process results in a considerably simpler and reliable method to load PS and other compounds. Also, after considering factors such as PS loading quantity, penetration in 3D bladder tumor organoids, and singlet oxygen production, the small UCNPs clusters displayed superior cell killing efficacy compared to single and big sized clusters. Therefore, these UCNPs clusters with different sizes could facilitate a clear and deep understanding of nanoparticle-based delivery platform systems for cell killing and may pave a new way for other fields of UCNPs based applications.

Citing Articles

NIR-triggered photooxygenation of α-terpinene with upconversion nanohybrids.

Frances-Soriano L, Bellezza D, Ferrera-Gonzalez J, Gonzalez-Bejar M, Perez-Prieto J Nanoscale Adv. 2024; .

PMID: 39355838 PMC: 11440474. DOI: 10.1039/d4na00528g.

Comparison of the Differences between Two-Photon Excitation, Upconversion, and Conventional Photodynamic Therapy on Cancers in In Vitro and In Vivo Studies.

Xu C, Law S, Leung A Pharmaceuticals (Basel). 2024; 17(6).

PMID: 38931331 PMC: 11206628. DOI: 10.3390/ph17060663.

Toxicity of Large and Small Surface-Engineered Upconverting Nanoparticles for In Vitro and In Vivo Bioapplications.

Machova Urdzikova L, Marekova D, Vasylyshyn T, Matous P, Patsula V, Oleksa V Int J Mol Sci. 2024; 25(10).

PMID: 38791332 PMC: 11121289. DOI: 10.3390/ijms25105294.

Wireless sequential dual light delivery for programmed PDT in vivo.

Liu J, Sun B, Li W, Kim H, Gan S, Ho J Light Sci Appl. 2024; 13(1):113.

PMID: 38744817 PMC: 11094163. DOI: 10.1038/s41377-024-01437-x.

Bladder microenvironment actuated proteomotors with ammonia amplification for enhanced cancer treatment.

Tian H, Ou J, Wang Y, Sun J, Gao J, Ye Y Acta Pharm Sin B. 2023; 13(9):3862-3875.

PMID: 37719374 PMC: 10501867. DOI: 10.1016/j.apsb.2023.02.016.

References

Mojzisova H, Bonneau S, Vever-Bizet C, Brault D . Cellular uptake and subcellular distribution of chlorin e6 as functions of pH and interactions with membranes and lipoproteins. Biochim Biophys Acta. 2007; 1768(11):2748-56. DOI: 10.1016/j.bbamem.2007.07.002. View

Wang F, Wen S, He H, Wang B, Zhou Z, Shimoni O . Microscopic inspection and tracking of single upconversion nanoparticles in living cells. Light Sci Appl. 2019; 7:18007. PMC: 5987356. DOI: 10.1038/lsa.2018.7. View

Bansal A, Yang F, Xi T, Zhang Y, Ho J . In vivo wireless photonic photodynamic therapy. Proc Natl Acad Sci U S A. 2018; 115(7):1469-1474. PMC: 5816188. DOI: 10.1073/pnas.1717552115. View

Li Z, Zhang Y . An efficient and user-friendly method for the synthesis of hexagonal-phase NaYF(4):Yb, Er/Tm nanocrystals with controllable shape and upconversion fluorescence. Nanotechnology. 2011; 19(34):345606. DOI: 10.1088/0957-4484/19/34/345606. View

Weijer R, Broekgaarden M, van Golen R, Bulle E, Nieuwenhuis E, Jongejan A . Low-power photodynamic therapy induces survival signaling in perihilar cholangiocarcinoma cells. BMC Cancer. 2015; 15:1014. PMC: 4691291. DOI: 10.1186/s12885-015-1994-2. View

Melissaridou S, Wiechec E, Magan M, Jain M, Chung M, Farnebo L . The effect of 2D and 3D cell cultures on treatment response, EMT profile and stem cell features in head and neck cancer. Cancer Cell Int. 2019; 19:16. PMC: 6332598. DOI: 10.1186/s12935-019-0733-1. View

Alberto M, Cuello H, Gulino C, Pifano M, Belgorosky D, Gabri M . Expression of bladder cancer-associated glycans in murine tumor cell lines. Oncol Lett. 2019; 17(3):3141-3150. PMC: 6396118. DOI: 10.3892/ol.2019.9995. View

Kwiatkowski S, Knap B, Przystupski D, Saczko J, Kedzierska E, Knap-Czop K . Photodynamic therapy - mechanisms, photosensitizers and combinations. Biomed Pharmacother. 2018; 106:1098-1107. DOI: 10.1016/j.biopha.2018.07.049. View

Cabral H, Matsumoto Y, Mizuno K, Chen Q, Murakami M, Kimura M . Accumulation of sub-100 nm polymeric micelles in poorly permeable tumours depends on size. Nat Nanotechnol. 2011; 6(12):815-23. DOI: 10.1038/nnano.2011.166. View

10.

Wan M, Lin J . Current evidence and applications of photodynamic therapy in dermatology. Clin Cosmet Investig Dermatol. 2014; 7:145-63. PMC: 4038525. DOI: 10.2147/CCID.S35334. View

11.

Amaral R, Miranda M, Marcato P, Swiech K . Comparative Analysis of 3D Bladder Tumor Spheroids Obtained by Forced Floating and Hanging Drop Methods for Drug Screening. Front Physiol. 2017; 8:605. PMC: 5572239. DOI: 10.3389/fphys.2017.00605. View

12.

Ivanov D, Parker T, Walker D, Alexander C, Ashford M, Gellert P . Multiplexing spheroid volume, resazurin and acid phosphatase viability assays for high-throughput screening of tumour spheroids and stem cell neurospheres. PLoS One. 2014; 9(8):e103817. PMC: 4131917. DOI: 10.1371/journal.pone.0103817. View

13.

Zhang D, Wei L, Zhong M, Xiao L, Li H, Wang J . The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy. Chem Sci. 2018; 9(23):5260-5269. PMC: 6001388. DOI: 10.1039/c8sc01828f. View

14.

Wang C, Cheng L, Liu Z . Drug delivery with upconversion nanoparticles for multi-functional targeted cancer cell imaging and therapy. Biomaterials. 2010; 32(4):1110-20. DOI: 10.1016/j.biomaterials.2010.09.069. View

15.

LaRue K, Khalil M, Freyer J . Microenvironmental regulation of proliferation in multicellular spheroids is mediated through differential expression of cyclin-dependent kinase inhibitors. Cancer Res. 2004; 64(5):1621-31. DOI: 10.1158/0008-5472.can-2902-2. View

16.

Mahalingam S, Ordaz J, Low P . Targeting of a Photosensitizer to the Mitochondrion Enhances the Potency of Photodynamic Therapy. ACS Omega. 2018; 3(6):6066-6074. PMC: 6045488. DOI: 10.1021/acsomega.8b00692. View

17.

Niculaes D, Lak A, Anyfantis G, Marras S, Laslett O, Avugadda S . Asymmetric Assembling of Iron Oxide Nanocubes for Improving Magnetic Hyperthermia Performance. ACS Nano. 2017; 11(12):12121-12133. PMC: 6097834. DOI: 10.1021/acsnano.7b05182. View

18.

Miao L, Newby J, Lin C, Zhang L, Xu F, Kim W . The Binding Site Barrier Elicited by Tumor-Associated Fibroblasts Interferes Disposition of Nanoparticles in Stroma-Vessel Type Tumors. ACS Nano. 2016; 10(10):9243-9258. PMC: 5515694. DOI: 10.1021/acsnano.6b02776. View

19.

Vasyutin I, Zerihun L, Ivan C, Atala A . Bladder Organoids and Spheroids: Potential Tools for Normal and Diseased Tissue Modelling. Anticancer Res. 2019; 39(3):1105-1118. DOI: 10.21873/anticanres.13219. View

20.

Svingen B, ONeal F, Aust S . The role of superoxide and singlet oxygen in lipid peroxidation. Photochem Photobiol. 1978; 28(4-5):803-9. DOI: 10.1111/j.1751-1097.1978.tb07022.x. View