Sensory Transduction in Escherichia Coli: Two Complementary Pathways of Information Processing That Involve Methylated Proteins

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Aug 1

PMID 333433

Citations 135

Authors

M S Springer

M F Goy

J Adler

Affiliations

Soon will be listed here.

Abstract

The properties of two classes of behavioral mutants of Escherichia coli (called tsr and tar) are described. The mutations in these strains define two complementary pathways of information flow in bacterial chemotaxis: behavioral responses to one set of stimuli are defective in tsr mutants, while responses to a complementary set of stimuli are defective in tar mutants. A double mutant containing both genetic lesions is defective in responses to all stimuli tested. The behavioral defects are shown to correlate with alterations in the properties of a methylation reaction involved in chemotaxis. Two independent sets of methyl-accepting proteins are demonstrated in the wild type, each set functioning in one of the two pathways mentioned above. Methylation of one set of proteins is defective in tsr mutants, while methylation of the complementary set is defective in tar mutants. The double mutant shows no methylation of either set. The relationship between the genetic loci (tsr and tar) and the methyl-accepting proteins is discussed.

Citing Articles

An chemoreceptor that mediates nitrate chemotaxis has conditional roles in the colonization of plant roots.

Ganusova E, Russell M, Patel S, Seats T, Alexandre G Appl Environ Microbiol. 2024; 90(6):e0076024.

PMID: 38775579 PMC: 11218637. DOI: 10.1128/aem.00760-24.

Substrate size-dependent conformational changes of bacterial pectin-binding protein crucial for chemotaxis and assimilation.

Anamizu K, Takase R, Hio M, Watanabe D, Mikami B, Hashimoto W Sci Rep. 2022; 12(1):12653.

PMID: 35879323 PMC: 9314435. DOI: 10.1038/s41598-022-16540-5.

Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, HM13.

Yokoyama F, Imai T, Aoki W, Ueda M, Kawamoto J, Kurihara T Front Microbiol. 2021; 12:629023.

PMID: 33679653 PMC: 7930318. DOI: 10.3389/fmicb.2021.629023.

Biphasic chemotaxis of to the microbiota metabolite indole.

Yang J, Chawla R, Rhee K, Gupta R, Manson M, Jayaraman A Proc Natl Acad Sci U S A. 2020; 117(11):6114-6120.

PMID: 32123098 PMC: 7084101. DOI: 10.1073/pnas.1916974117.

Molecular Mechanism for Attractant Signaling to DHMA by E. coli Tsr.

Orr A, Yang J, Sule N, Chawla R, Hull K, Zhu M Biophys J. 2019; 118(2):492-504.

PMID: 31839263 PMC: 6976796. DOI: 10.1016/j.bpj.2019.11.3382.

References

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Silverman M, Matsumura P, Hilmen M, Simon M . Characterization of lambda Escherichia coli hybrids carrying chemotaxis genes. J Bacteriol. 1977; 130(2):877-87. PMC: 235294. DOI: 10.1128/jb.130.2.877-887.1977. View

Silverman M, Simon M . Chemotaxis in Escherichia coli: methylation of che gene products. Proc Natl Acad Sci U S A. 1977; 74(8):3317-21. PMC: 431545. DOI: 10.1073/pnas.74.8.3317. View

Silverman M, Simon M . Identification of polypeptides necessary for chemotaxis in Escherichia coli. J Bacteriol. 1977; 130(3):1317-25. PMC: 235356. DOI: 10.1128/jb.130.3.1317-1325.1977. View

Springer W, Koshland Jr D . Identification of a protein methyltransferase as the cheR gene product in the bacterial sensing system. Proc Natl Acad Sci U S A. 1977; 74(2):533-7. PMC: 392324. DOI: 10.1073/pnas.74.2.533. View

Warrick H, Taylor B, Koshland Jr D . Chemotactic mechanism of Salmonella typhimurium: preliminary mapping and characterization of mutants. J Bacteriol. 1977; 130(1):223-31. PMC: 235197. DOI: 10.1128/jb.130.1.223-231.1977. View

Springer M, Goy M, Adler J . Sensory transduction in Escherichia coli: a requirement for methionine in sensory adaptation. Proc Natl Acad Sci U S A. 1977; 74(1):183-7. PMC: 393222. DOI: 10.1073/pnas.74.1.183. View

PARKINSON J . Genetics of chemotactic behavior in bacteria. Cell. 1975; 4(3):183-8. DOI: 10.1016/0092-8674(75)90166-x. View

Tso W, Adler J . Negative chemotaxis in Escherichia coli. J Bacteriol. 1974; 118(2):560-76. PMC: 246789. DOI: 10.1128/jb.118.2.560-576.1974. View

10.

Adler J, Hazelbauer G, Dahl M . Chemotaxis toward sugars in Escherichia coli. J Bacteriol. 1973; 115(3):824-47. PMC: 246327. DOI: 10.1128/jb.115.3.824-847.1973. View

11.

Adler J . A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973; 74(1):77-91. DOI: 10.1099/00221287-74-1-77. View

12.

Tsang N, Macnab R, Koshland Jr D . Common mechanism for repellents and attractants in bacterial chemotaxis. Science. 1973; 181(4094):60-3. DOI: 10.1126/science.181.4094.60. View

13.

Adler J, Tso W . "Decision"-making in bacteria: chemotactic response of Escherichia coli to conflicting stimuli. Science. 1974; 184(4143):1292-4. DOI: 10.1126/science.184.4143.1292. View

14.

Mesibov R, Adler J . Chemotaxis toward amino acids in Escherichia coli. J Bacteriol. 1972; 112(1):315-26. PMC: 251414. DOI: 10.1128/jb.112.1.315-326.1972. View

15.

Macnab R, Koshland Jr D . The gradient-sensing mechanism in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1972; 69(9):2509-12. PMC: 426976. DOI: 10.1073/pnas.69.9.2509. View

16.

Hazelbauer G, Mesibov R, Adler J . Escherichia coli mutants defective in chemotaxis toward specific chemicals. Proc Natl Acad Sci U S A. 1969; 64(4):1300-7. PMC: 223283. DOI: 10.1073/pnas.64.4.1300. View

17.

Spudich J, Koshland Jr D . Quantitation of the sensory response in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1975; 72(2):710-3. PMC: 432385. DOI: 10.1073/pnas.72.2.710. View

18.

Adler J . Chemotaxis in bacteria. Annu Rev Biochem. 1975; 44:341-56. DOI: 10.1146/annurev.bi.44.070175.002013. View

19.

Kort E, Goy M, Larsen S, Adler J . Methylation of a membrane protein involved in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1975; 72(10):3939-43. PMC: 433112. DOI: 10.1073/pnas.72.10.3939. View

20.

Laskey R, Mills A . Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975; 56(2):335-41. DOI: 10.1111/j.1432-1033.1975.tb02238.x. View