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Simultaneous Incorporation of PTH(1-34) and Nano-hydroxyapatite into Chitosan/Alginate Hydrogels for Efficient Bone Regeneration

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Journal Bioact Mater
Date 2020 Dec 18
PMID 33336115
Citations 35
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Abstract

Tissue regeneration based on the utilization of artificial soft materials is considered a promising treatment for bone-related diseases. Here, we report cranial bone regeneration promoted by hydrogels that contain parathyroid hormone (PTH) peptide PTH(1-34) and nano-hydroxyapatite (nHAP). A combination of the positively charged natural polymer chitosan (CS) and negatively charged sodium alginate led to the formation of hydrogels with porous structures, as shown by scanning electron microscopy. Rheological characterizations revealed that the mechanical properties of the hydrogels were almost maintained upon the addition of nHAP and PTH(1-34). In vitro experiments showed that the hydrogel containing nHAP and PTH(1-34) exhibited strong biocompatibility and facilitated osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) via the Notch signaling pathway, as shown by the upregulated expression of osteogenic-related proteins. We found that increasing the content of PTH(1-34) in the hydrogels resulted in enhanced osteogenic differentiation of BMSCs. Implantation of the complex hydrogel into a rat cranial defect model led to efficient bone regeneration compared to the rats treated with the hydrogel alone or with nHAP, indicating the simultaneous therapeutic effect of nHAP and PTH during the treatment process. Both the in vitro and in vivo results demonstrated that simultaneously incorporating nHAP and PTH into hydrogels shows promise for bone regeneration, suggesting a new strategy for tissue engineering and regeneration in the future.

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References
1.
Jiang L, Zhang W, Wei L, Zhou Q, Yang G, Qian N . Early effects of parathyroid hormone on vascularized bone regeneration and implant osseointegration in aged rats. Biomaterials. 2018; 179:15-28. DOI: 10.1016/j.biomaterials.2018.06.035. View

2.
Pettway G, Meganck J, Koh A, Keller E, Goldstein S, McCauley L . Parathyroid hormone mediates bone growth through the regulation of osteoblast proliferation and differentiation. Bone. 2008; 42(4):806-18. PMC: 2677418. DOI: 10.1016/j.bone.2007.11.017. View

3.
Danoux C, Barbieri D, Yuan H, de Bruijn J, van Blitterswijk C, Habibovic P . In vitro and in vivo bioactivity assessment of a polylactic acid/hydroxyapatite composite for bone regeneration. Biomatter. 2014; 4:e27664. PMC: 3961484. DOI: 10.4161/biom.27664. View

4.
Stratton S, Shelke N, Hoshino K, Rudraiah S, Kumbar S . Bioactive polymeric scaffolds for tissue engineering. Bioact Mater. 2017; 1(2):93-108. PMC: 5482547. DOI: 10.1016/j.bioactmat.2016.11.001. View

5.
Sun J, Zhao X, Illeperuma W, Chaudhuri O, Oh K, Mooney D . Highly stretchable and tough hydrogels. Nature. 2012; 489(7414):133-6. PMC: 3642868. DOI: 10.1038/nature11409. View