A Genome Engineering Resource to Uncover Principles of Cellular Organization and Tissue Architecture by Lipid Signaling

Overview

Journal Elife

Specialty Biology

Date 2020 Dec 15

PMID 33320085

Citations 9

Authors

Deepti Trivedi

Vinitha Cm

Karishma Bisht

Vishnu Janardan

Awadhesh Pandit

Bishal Basak

Shwetha H

Navyashree Ramesh

Padinjat Raghu

Affiliations

Soon will be listed here.

Abstract

Phosphoinositides (PI) are key regulators of cellular organization in eukaryotes and genes that tune PI signaling are implicated in human disease mechanisms. Biochemical analyses and studies in cultured cells have identified a large number of proteins that can mediate PI signaling. However, the role of such proteins in regulating cellular processes and development in metazoans remains to be understood. Here, we describe a set of CRISPR-based genome engineering tools that allow the manipulation of each of these proteins with spatial and temporal control during metazoan development. We demonstrate the use of these reagents to deplete a set of 103 proteins individually in the eye and identify several new molecules that control eye development. Our work demonstrates the power of this resource in uncovering the molecular basis of tissue homeostasis during normal development and in human disease biology.

Citing Articles

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