Long Non-Coding RNA Accelerates the Progression of Colorectal Cancer Through / Axis

Overview

Journal Cancer Manag Res

Publisher Dove Medical Press

Specialty Oncology

Date 2020 Dec 14

PMID 33312000

Citations 9

Authors

Chuanping Yuan

Ling Yang

Affiliations

Soon will be listed here.

Abstract

Background: Long non-coding RNAs (lncRNAs) are essential regulators in colorectal cancer (CRC) progression. This work aimed to delve into the characteristics of lncRNA in CRC.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was adopted to examine , high-mobility group box-1 (HMGB1) mRNA expressions in CRC tissues and cell lines. Functionally, cell counting kit-8 (CCK-8) and 5-ethynyl-2-deoxyuridine (EdU) incorporation assays were employed to examine cell proliferation; wound healing assay was utilized to detect cell migration; and flow cytometry was used to detect the cell apoptosis. Luciferase reporter assay, RNA immunoprecipitation assay and Western blot were conducted to detect the regulatory relationships among and HMGB1.

Results: and HMGB1 mRNA expressions were observably elevated in CRC tissues and cell lines. Knocking down could significantly inhibit cell proliferation and migration, and promote apoptosis of CRC cells. could serve as a competitive endogenous RNA, and up-regulate HMGB1 expression by adsorbing .

Conclusion: can expedite CRC cell proliferation and migration, and inhibit apoptosis through /HMGB1 axis.

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References

Chen X, Liu X, He B, Pan Y, Sun H, Xu T . MiR-216b functions as a tumor suppressor by targeting HMGB1-mediated JAK2/STAT3 signaling way in colorectal cancer. Am J Cancer Res. 2017; 7(10):2051-2069. PMC: 5665852. View

Sun J, Zhang Y, Li B, Dong Y, Sun C, Zhang F . PITPNA-AS1 abrogates the inhibition of miR-876-5p on WNT5A to facilitate hepatocellular carcinoma progression. Cell Death Dis. 2019; 10(11):844. PMC: 6838072. DOI: 10.1038/s41419-019-2067-2. View

Kuniyasu H, Chihara Y, Kondo H . Differential effects between amphoterin and advanced glycation end products on colon cancer cells. Int J Cancer. 2003; 104(6):722-7. DOI: 10.1002/ijc.11016. View

He S, Cheng J, Sun L, Wang Y, Wang C, Liu X . HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect. Cell Death Dis. 2018; 9(6):648. PMC: 5974346. DOI: 10.1038/s41419-018-0626-6. View

Wu Q, Meng W, Jie Y, Zhao H . LncRNA MALAT1 induces colon cancer development by regulating miR-129-5p/HMGB1 axis. J Cell Physiol. 2017; 233(9):6750-6757. DOI: 10.1002/jcp.26383. View

Jin X, Hong Y, Li P, Hao L, Chen M . Long noncoding RNA LINC00520 accelerates the progression of colorectal cancer by serving as a competing endogenous RNA of microRNA-577 to increase HSP27 expression. Hum Cell. 2020; 33(3):683-694. DOI: 10.1007/s13577-020-00336-8. View

Hirata Y, Kurobe H, Higashida M, Fukuda D, Shimabukuro M, Tanaka K . HMGB1 plays a critical role in vascular inflammation and lesion formation via toll-like receptor 9. Atherosclerosis. 2013; 231(2):227-33. DOI: 10.1016/j.atherosclerosis.2013.09.010. View

Zhang W, An F, Xia M, Zhan Q, Tian W, Jiao Y . Increased HMGB1 expression correlates with higher expression of c-IAP2 and pERK in colorectal cancer. Medicine (Baltimore). 2019; 98(3):e14069. PMC: 6370169. DOI: 10.1097/MD.0000000000014069. View

Tripathi A, Shrinet K, Kumar A . HMGB1 protein as a novel target for cancer. Toxicol Rep. 2019; 6:253-261. PMC: 6416660. DOI: 10.1016/j.toxrep.2019.03.002. View

10.

Khorkova O, Hsiao J, Wahlestedt C . Basic biology and therapeutic implications of lncRNA. Adv Drug Deliv Rev. 2015; 87:15-24. PMC: 4544752. DOI: 10.1016/j.addr.2015.05.012. View

11.

Siegel R, Miller K, Sauer A, Fedewa S, Butterly L, Anderson J . Colorectal cancer statistics, 2020. CA Cancer J Clin. 2020; 70(3):145-164. DOI: 10.3322/caac.21601. View

12.

Brenner H, Chen C . The colorectal cancer epidemic: challenges and opportunities for primary, secondary and tertiary prevention. Br J Cancer. 2018; 119(7):785-792. PMC: 6189126. DOI: 10.1038/s41416-018-0264-x. View

13.

Dacheng W, Songhe L, Weidong J, Shutao Z, Jingjing L, Jiaming Z . RETRACTED: LncRNA SNHG3 promotes the growth and metastasis of colorectal cancer by regulating miR-539/RUNX2 axis. Biomed Pharmacother. 2020; 125:110039. DOI: 10.1016/j.biopha.2020.110039. View

14.

Jin Z, Chen B . LncRNA ZEB1-AS1 Regulates Colorectal Cancer Cells by MiR-205/YAP1 Axis. Open Med (Wars). 2020; 15:175-184. PMC: 7065425. DOI: 10.1515/med-2020-0026. View

15.

Ambros V . microRNAs: tiny regulators with great potential. Cell. 2002; 107(7):823-6. DOI: 10.1016/s0092-8674(01)00616-x. View

16.

Wang Q, Yu J . MiR-129-5p suppresses gastric cancer cell invasion and proliferation by inhibiting COL1A1. Biochem Cell Biol. 2017; 96(1):19-25. DOI: 10.1139/bcb-2016-0254. View

17.

Tang D, Loze M, Zeh H, Kang R . The redox protein HMGB1 regulates cell death and survival in cancer treatment. Autophagy. 2010; 6(8):1181-3. DOI: 10.4161/auto.6.8.13367. View

18.

Balacescu O, Sur D, Cainap C, Visan S, Cruceriu D, Manzat-Saplacan R . The Impact of miRNA in Colorectal Cancer Progression and Its Liver Metastases. Int J Mol Sci. 2018; 19(12). PMC: 6321452. DOI: 10.3390/ijms19123711. View

19.

Salmena L, Poliseno L, Tay Y, Kats L, Pandolfi P . A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language?. Cell. 2011; 146(3):353-8. PMC: 3235919. DOI: 10.1016/j.cell.2011.07.014. View

20.

Van der Valk M, Hilling D, Bastiaannet E, Meershoek-Klein Kranenbarg E, Beets G, Figueiredo N . Long-term outcomes of clinical complete responders after neoadjuvant treatment for rectal cancer in the International Watch & Wait Database (IWWD): an international multicentre registry study. Lancet. 2018; 391(10139):2537-2545. DOI: 10.1016/S0140-6736(18)31078-X. View