Ultrasensitive TiCT MXene/Chitosan Nanocomposite-Based Amperometric Biosensor for Detection of Potential Prostate Cancer Marker in Urine Samples

Overview

Journal Processes (Basel)

Date 2020 Dec 11

PMID 33304843

Citations 14

Authors

Stefania Hroncekova

Tomas Bertok

Michal Hires

Eduard Jane

Lenka Lorencova

Alica Vikartovska

Aisha Tanvir

Peter Kasak

Jan Tkac

Affiliations

Soon will be listed here.

Abstract

Two-dimensional layered nanomaterial TiCT (a member of the MXene family) was used to immobilise enzyme sarcosine oxidase to fabricate a nanostructured biosensor. The device was applied for detection of sarcosine, a potential prostate cancer biomarker, in urine for the first time. The morphology and structures of MXene have been characterised by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Electrochemical measurements, SEM and AFM analysis revealed that MXene interfaced with chitosan is an excellent support for enzyme immobilisation to fabricate a sensitive biosensor exhibiting a low detection limit of 18 nM and a linear range up to 7.8 µM. The proposed biosensing method also provides a short response time of 2 s and high recovery index of 102.6% for detection of sarcosine spiked into urine sample in a clinically relevant range.

Citing Articles

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