Amplified Fragment Length Polymorphism: Applications and Recent Developments

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2020 Dec 10

PMID 33301096

Citations 6

Authors

Thotten Elampilay Sheeja

Illathidath Payatatti Vijesh Kumar

Ananduchandra Giridhari

Divakaran Minoo

Muliyar Krishna Rajesh

Kantipudi Nirmal Babu

Affiliations

Soon will be listed here.

Abstract

AFLP or amplified fragment length polymorphism is a PCR-based molecular technique that uses selective amplification of a subset of digested DNA fragments from any source to generate and compare unique fingerprints of genomes. It is more efficient in terms of time, economy, reproducibility, informativeness, resolution, and sensitivity, compared to other popular DNA markers. Besides, it requires very small quantities of DNA and no prior genome information. This technique is widely used in plants for taxonomy, genetic diversity, phylogenetic analysis, construction of high-resolution genetic maps, and positional cloning of genes, to determine relatedness among cultivars and varietal identity, etc. The review encompasses in detail the various applications of AFLP in plants and the major advantages and disadvantages. The review also considers various modifications of this technique and novel developments in detection of polymorphism. A wet-lab protocol is also provided.

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